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Merck

PMEF-NL

EmbryoMax® Primary Mouse Embryonic Fibroblasts

Synonyma:

MEF Feeder Cells, neo PMEFs, neo MEFs, MEFs, Mouse Feeder Cells

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O této položce

UNSPSC Code:
41106509
NACRES:
NA.81
eCl@ss:
32011203

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biological source

mouse

manufacturer/tradename

Specialty Media
EmbryoMax®

technique(s)

cell culture | stem cell: suitable

input

sample type primary embryotic fibroblasts (PMEFs)
sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)

shipped in

liquid nitrogen

Quality Level

100

Physical form

The cells are provided frozen into individual vials, containing approximately 5-6 x 106 fibroblasts per vial, and are sold as a pack of 5 vials and supplied at passage 3.

Preparation Note

After receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.

Legal Information

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Application

PMEF, Neo Resistant, Strain FVB, Not Treated, Passage 3

Biochem/physiol Actions

Primary Mouse Embryo Fibroblasts

General description

Primary Mouse Embryo Fibroblasts, Neo resistant, strain FVB, are not Mytomycin C treated and serve as a feeder layer for both mouse and human ES/IPS cells. MEF cells are resistant to neomycin. The cells are provided at passage 3 and can be further expanded before freezing for later use.

Plating MEF Feeder Cells

Procedure:

1. Prior to thawing PMEF feeder cells, coat plates/flasks with Gelatin solution.
2. Thaw PMEF vial(s) quickly in a 37 °C water bath and transfer to a 15 mL tube (already containing 10 mL of warm PMEF Feeder Cell Medium). Gently invert the tube to distribute, and centrifuge at 300 xg for 4–5 minutes.
3. Remove supernatant and resuspend the cell pellet in warm PMEF Feeder Cell Medium.
4. Remove the Gelatin solution from plates/flasks, and aliquot the PMEF feeder cell suspension at the densities recommended in Table 4.1 of the mouse ES protocol guide
5. Incubate the PMEF Feeder cells at 37 °C with 5% CO2. Use Figures 4A, B and C in the mouse ES protocol guide as a guide for an estimate of correct PMEF density and
appearance. Gelatinized plates may be used for 12–14 days.
The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.

Packaging

5-6x106 ea

Skladovací třída

12 - Non Combustible Liquids

flash_point_f

does not flash

flash_point_c

does not flash

Osvědčení o analýze (COA)

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Sortimentní položky

Myší embryonální fibroblasty (MEF Feeder Cells)

Myší embryonální fibroblasty (MEF) slouží jako živná vrstva pro myší i lidské embryonální kmenové buňky (ES buňky) a indukované pluripotentní kmenové buňky (iPSC).

Mouse Embryonic Fibroblasts (MEF Feeder Cells)

Mouse embryonic fibroblasts (MEFs) serve as a feeder layer for both mouse and human embryonic stem cells (ES cells) and induced pluripotent stem cells (iPSCs).

Protokoly

Mouse Embryonic Stem Cell Culture Procedures & Protocols

Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.

Související obsah

Murine Embryonic Stem Cell Culture Procedures & Protocols

The cell culture protocols described in this instructional manual include the in vitro culture of murine ES cells using EmbryoMax products along with ESGRO® mLIF medium supplement, as well as feeder-free and serum-free ES cell culture using the ESGRO Complete™ line of products. Also included in this instructional manual are: methods for serum-free neuronal differentiation of mouse ES cells, iPS cell generation using STEMCCA™ lentivirus kits, cre-recombinase mediated excision of STEMCCA™ genes from iPS cells, derivation and rescue of new and existing ES cell lines using RESGRO™ Culture Medium and ESGRO Complete™ system.

Plating PMEF Feeder Cells Protocol
The Genetically Modified Mouse: An Indispensable Tool for Disease Modeling

"As transgenic mouse core laboratories know well, mouse models are a powerful means of determining the function of mammalian genes in the context of whole organisms—especially since over 95% of the mouse genome is similar to the human genome. However, you may not know that: 1. Laboratory mouse strains are descended from ancestors in modern-day Pakistan. 2. The first inbred strain of lab mouse was created in 1909. It was named DBA for its coat color: dilute brown non-agouti. 3. There is a mutant mouse actually named“Bad Hair Day” (Bhrd). Someone get this little guy a stylist! 4. Male mice, when treated with anticancer drugs, have been shown to pass on DNA instability to their offspring. 5. The founder of Jackson Laboratories, C. C. Little, once had lunch with Walt Disney to request that an animated film be created linking Mickey Mouse and the laboratory mouse. The request was denied."

Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

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