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Key Documents

MABC008

Sigma-Aldrich

Mouse IgM Negative Control, clone GC323

Mouse IgM Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.42

biological source

mouse

Quality Level

clone

GC323, monoclonal

manufacturer/tradename

Chemicon®

concentration

0.5 mg/mL

technique(s)

flow cytometry: suitable
immunofluorescence: suitable

isotype

IgM

shipped in

wet ice

target post-translational modification

unmodified

Specificity

This antibody reacts specifically with the outer membrane protein I component of various strains of Neisseria gonorrhoeae and does not react with any human cell surface component.

Immunogen

Protein I preparation of Neisseria gonorrhoeae.

Application

Research Category
Secondary & Control Antibodies

Epitope Tags & General Use
Research Sub Category
Isotype Control Antibodies
The use of this antibody enables an estimation of non-specific binding of mouse monoclonal antibodies of isotype IgM to cell surface components in human blood and bone marrow. It is not suitable for use in immunohistochemical procedures.

SUGGESTED USAGE

The control antibody should be diluted to the same concentration as the test antibody, and equivalent volumes used. Flow cytometry and immunofluorescence - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.

Physical form

Format: Purified
Purified from mouse ascitic fluid. The antibody is supplied in 0.5ml 25mM Tris HCl, 0.4M NaCl, pH 8.0, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis, specific immunofluorescence assay and flow cytometry.

Storage and Stability

Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

8B - Non-combustible corrosive hazardous materials

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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