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Key Documents

MAB5256A5

Sigma-Aldrich

Anti-Neurofilament 200kDa Antibody, clone NE14, Alexa Fluor™ 555 Conjugate

clone NE14, from mouse, ALEXA FLUOR™ 555

Synonyma:

Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR™ 555

antibody form

purified antibody

antibody product type

primary antibodies

clone

NE14, monoclonal

species reactivity

mouse, rat, pig

species reactivity (predicted by homology)

human (100% sequence homology), porcine (immunogen homology)

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable

isotype

IgG1

NCBI accession no.

NP_066554

UniProt accession no.

P12036

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NEFH(4744)
mouse ... Nefh(380684)
pig ... Nefh(100156492)
rat ... Nefh(24587)

General description

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information and epitope maps, search "neurofilament" on our website.

Application

Detect Neurofilament 200kDa using this Anti-Neurofilament 200kDa Antibody, clone NE14, Alexa Fluor 555 Conjugate validated for use in ICC & IHC.

Quality

Evaluated by Immunocytochemistry in rat E18 primary cortex cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Neurofilament in rat E18 primary cortex cells.

Other Notes

Unconjugated Anti-Neurofilament has been shown to work with multiple species including Human and Porcine.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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