MAB1614

Anti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2

clone H2, Chemicon^®, from mouse

• Synonyma: Anti-HEL-S-61, Anti-KINH, Anti-KNS, Anti-KNS1, Anti-UKHC
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Vlastnosti

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: H2, monoclonal
• species reactivity: sea urchin, rat, chicken, squid, human, mouse
• species reactivity (predicted by homology): mammals
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; radioimmunoassay: suitable; western blot: suitable
• isotype: IgG2b
• NCBI accession no.: NP_004512
• UniProt accession no.: P33176
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... KIF5B(3799)

Popis

General description

Kinesins are molecular motors used for intracellular transport and trafficking. They move along microtubule cables powered by the hydrolysis of ATP, thus kinesins are ATPases. The active movement of kinesins supports several cellular functions including mitosis, meiosis and transport of cargo such as axonal transport.

Specificity

Reacts with the heavy chain of kinesin near the N-terminus.

Immunogen

Bovine brain kinesin.

Epitope: a.a.420-445

Application

Detect Kinesin using this Anti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2 validated for use in ELISA, IC, IH, IP, FUNC, RIA & WB.

Immunohistochemistry:
A previous lot was used on paraformaldehyde fixed tissue. Also works for EM.

Immunocytochemistry:
A previous lot of this antibody was used in Immunocytochemistry.

Immunoprecipitation:
A previous lot of this antibody was used in Immunoprecipitation.

EIA/RIA:
A previous lot of this antibody was used in RIA.

Immunoblotting: recognizes 124kDa KHC and its proteolytic fragments.

Inhibition of kinesin mediated transport {Brady, et al PNAS 87(3):1061-1065, 1990.

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Quality

Routinely evaluated by Western Blot on Mouse Brain lysates.

Western Blot Analysis:
1:500 dilution of this lot detected KINESIN (HEAVY CHAIN) on 10 μg of Mouse Brain lysates.

Target description

124 kDa

Physical form

Format: Purified

Protein A purified

Purified mouse monoclonal IgG2b in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide.

Storage and Stability

Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.

Analysis Note

Control
Brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Bezpečnostní informace

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable