GF048

Macrophage Inflammatory Protein-1α Protein, Recombinant rat

• UNSPSC Code: 12352202
• NACRES: NA.28
• eCl@ss: 32160405

Vlastnosti

• assay: >95% (SDS-PAGE and HPLC)
• technique(s): cell culture | mammalian: suitable
• UniProt accession no.: P10147 ; P50229
• Quality Level: 100

Popis

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

FUNCTION: SwissProt: P10147 # Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
SIZE: 92 amino acids; 10085 Da
SUBUNIT: Self-associates. Also heterodimer of MIP-1-alpha(4-69) and MIP-1-beta(3-69).
SUBCELLULAR LOCATION: Secreted.
PTM: N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T- cells.
SIMILARITY: SwissProt: P10147 ## Belongs to the intercrine beta (chemokine CC) family.

GF048 produces chemotaxis over a wide concentration range in an assay using rat peritoneal macrophages. Significant chemotaxis was achieved using a concentration of 50-100 ng/mL.

Physical form

Lyophilized powder with no additives.

Preparation Note

The lyophilized powder, though stable at room temperature, is best stored at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Further dilutions can be made into other aqueous buffers and stored at 2-8 °C for 1 week or -20 °C for future use. Reconstituted rat MIP-1alpha should be stored in undiluted aliquots at -20°C for up to 6 months.

Bezpečnostní informace

• Skladovací třída: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable