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Merck

EZHGIP

Human GIP ELISA Kit

measures and quantify total GIP levels in 20 μL serum, plasma or cell culture samples

Synonyma:

Gastric inhibitory polypeptide, Glucose-dependent insulinotropic polypeptide, Incretin hormone

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Vybrat velikost

96 WELLS

18 900,00 Kč

10 X 96 WELL

132 800,00 Kč

18 900,00 Kč


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O této položce

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.32
Gene information:
human ... GNAI2(2771)
Input:
sample type cell culture supernatant
sample type serum
sample type plasma (K2 EDTA)
Assay range:
accuracy: 86.7%, linearity: 99.9%, sensitivity: 8.2 pg/mL, standard curve range: 8.2-2000 pg/mL
Species reactivity:
human
Storage temp.:
2-8°C
Shipped in:
wet ice

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Název produktu

Human GIP (total) ELISA, This Human GIP (total) ELISA is used to measure & quantify GIP levels in Metabolism & Endocrine research.

Quality Level

species reactivity

human

packaging

kit of 1 × 96 wells

parameter

20 μL sample volume (4hr assay)

assay range

accuracy: 86.7%, linearity: 99.9%, sensitivity: 8.2 pg/mL, standard curve range: 8.2-2000 pg/mL

technique(s)

ELISA: suitable

input

sample type cell culture supernatant
sample type serum
sample type plasma (K2 EDTA)

NCBI accession no.

UniProt accession no.

application(s)

research use

detection method

colorimetric (450nm/590nm)

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... GNAI2(2771)

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Tato položka
EZRMGIPEZGRTEZHI
species reactivity

human

species reactivity

mouse, rat

species reactivity

human

species reactivity

human

Gene Information

human ... GNAI2(2771)

Gene Information

-

Gene Information

-

Gene Information

-

assay range

accuracy: 86.7%, sensitivity: 8.2 pg/mL, linearity: 99.9%, standard curve range: 8.2-2000 pg/mL

assay range

accuracy: 104.2%, linearity: 100.8%, sensitivity: 8.2 pg/mL, standard curve range: 8.2-2000 pg/mL

assay range

accuracy: 101-107%
(Serum: Des-octanoyl Ghrelin), accuracy: 88-91%
(Serum: Active Ghrelin), linearity: 95-117%
(plasma), linearity: 95.7-104.7%
(serum), sensitivity: 156 pg/mL
(20 µL Sample Size), inter-assay cv: 5.2-7.7%
intra-assay cv: 1.1-1.9%
(Plasma), inter-assay cv: 6.2-7.8%
intra-assay cv: 0.9-1.3%
(Serum)

assay range

accuracy: 76-103%, linearity: 96-112%, standard curve range: 2-200 μU/mL

input

sample type cell culture supernatant
sample type serum
sample type plasma (K2 EDTA)

input

sample type cell culture supernatant
sample type plasma (K2 EDTA)
sample type serum

input

sample type plasma (K2 EDTA)
sample type serum

input

sample type plasma (K2 EDTA)
sample type serum
sample type cell culture supernatant

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

General description

GIP, also known as a gastric inhibitory polypeptide, or glucose-dependent insulinotropic polypeptide is encoded by the gene mapped to human chromosome 17q12-q21. It is synthesized in and secreted from K cells in the intestinal epithelium. GIP secretion from the gastrointestinal tract plays a major role in glucose homeostasis. It stimulates insulin secretion from pancreatic β cells after oral ingestion of nutrients. In addition, GIP also promotes insulin biosynthesis, and β-cell proliferation and alleviates beta-cell apoptosis GIP also aids in fat metabolism by stimulating triglyceride synthesis in adipocytes. Elevated levels of GIP may lead to obesity and hyperinsulinemia. This Human GIP (total) ELISA is used to measure & quantify GIP levels in Metabolism & Endocrine research.

Application

Human GIP (total) ELISA has been used to measure total glucose-dependent insulinotropic polypeptide (GIP) in blood samples.

Other Notes

Research Sub Category Obesity Metabolic Disorders Room temperature, 3.5 hour assay, 20 µL sample volume, serum, plasma, or tissue culture medium Research Category Metabolism

Disclaimer

For research use only. Not for use in diagnostic procedures.

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Met. Corr. 1 - Skin Sens. 1

Skladovací třída

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3


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Navštívit knihovnu dokumentů

Tsuyoshi Yanagimachi et al.
Molecular metabolism, 6(2), 226-231 (2017-02-10)
Glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) possess multiple bioactive isoforms that are rendered non-insulinotropic by the enzyme dipeptidyl peptidase-4 (DPP-4). Recently, some ELISA kits have been developed to specifically measure "active" GIP and GLP-1, but it is unclear
L A Anderson et al.
Genomics, 17(3), 618-623 (1993-09-01)
To facilitate the positional cloning of the breast-ovarian cancer gene BRCA1, we constructed a high-density genetic map of the 8.3-cM interval between D17S250 and GIP on chromosome 17q12-q21. Markers were mapped by linkage in the CEPH and in extended kindreds
Colesevelam improves oral but not intravenous glucose tolerance by a mechanism independent of insulin sensitivity and ?-cell function.
Anna L Marina,Kristina M Utzschneider,Lorena A Wright,Brenda K Montgomery et al.
Diabetes Care null
Dietary sugars stimulate fatty acid synthesis in adults.
Elizabeth J Parks, Lauren E Skokan, Maureen T Timlin, Carlus S Dingfelder
The Journal of Nutrition null
Frank Isken et al.
American journal of physiology. Endocrinology and metabolism, 295(2), E350-E355 (2008-05-29)
Menopause and premature gonadal steroid deficiency are associated with increases in fat mass and body weight. Ovariectomized (OVX) mice also show reduced locomotor activity. Glucose-dependent-insulinotropic-polypeptide (GIP) is known to play an important role both in fat metabolism and locomotor activity.

Globální číslo obchodní položky

Skladová položkaGTIN
EZHGIP-54K04053252736346
EZHGIP-54BK04053252436956

Questions

1–2 of 2 Questions  
  1. What is the sample type? Urine?

    1 answer
    1. This product has been qualified for use with serum or plasma samples. The end user would have to determine suitability with urine.

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  2. What is the CAS numbers for this product - it does not appear on the SDS

    1 answer
    1. This item is a multi-component product. A CAS number cannot be applied to this type of product. The Safety Data Sheet document includes the individual CAS numbers of those components that may be potentially hazardous.

      Helpful?

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