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AM80

Sigma-Aldrich

Anti-PAR (Ab-1) Mouse mAb (10H)

liquid, ≥95% (SDS-PAGE), clone 10H

Synonyma:

Anti-Poly (ADP-Ribose)

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

10H, monoclonal

assay

≥95% (SDS-PAGE)

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, Drosophila, rat, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG3

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Protein A purified mouse monoclonal antibody. Recognizes poly-ADP-ribose.
Recognizes poly(ADP)-ribose (PAR) in etopside-treated HL-60 cells.
This Anti-PAR (Ab-1) Mouse mAb (10H) is validated for use in Immunoblotting, Immunocytochemistry for the detection of PAR (Ab-1).

Immunogen

Bovine
purified poly(ADP-ribose)

Application

Immunoblotting (2.5 µg/ml)

Immunocytochemistry (5-20 µg/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 100 mM NaCl, 50 mM HEPES, 1% BSA, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
Normal mouse IgG
Positive Control
Etoposide-treated HL-60 cells

Other Notes

Kawamitsu, H., et al. 1984. Biochemistry23, 3771.
PAR can be induced in HL-60 cells by treatment with 67 µM etoposide for 3.5 h. Antibody should be titrated for optimal results in an individual system.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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