ABS1026
Anti-Rab27B Antibody
serum, from rabbit
Synonyma:
Ras-related protein Rab-27B, C25KG
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Doporučené produkty
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, rat, mouse
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... RAB27B(5874)
General description
RAB27B, also known as C25KG, is a member of the Rab GTPase superfamily. RAB27B acts as a lipid anchoring protein that is prenylated and works in vesicular fusion and protein trafficking within cells. The protein is most highly expressed in testis but can be expressed in other cell types as well. Because Rab27B is widely expressed in various tissues together with Rab27A and RAB27B has been found to have the ability to bind all of the Rab27A effectors that have been tested, Rab27A and Rab27B were initially thought to function redundantly by sharing common Rab27 effectors. However, recent evidence has indicated that by interacting with different Rab27 effectors Rab27A and Rab27B play different roles in special types of secretion (e.g. exosome secretion and mast cell secretion) even within the same cell type. Rab27B is particularly important for regulating protein secretion by pancreatic acinar cells, for instance.
Immunogen
GST-tagged recombinant protein corresponding to human Rab27B.
Application
Research Category
Signaling
Signaling
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
This Anti-Rab27B Antibody is validated for use in Western Blotting and Immunoprecipitation and Immunohistochemistry for the detection of Rab27B.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Rab27B in 10 µg of C2C12, mouse brain, and PC3 lysate.
Western Blotting Analysis: A representative lot detected Rab27B in rat parotid gland lysate (Imai, A., et al. (2004). Journal of Cell Science. 117:1945-1953).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Rab27B in flg-Rab27B (Imai, A., et al. (2004). Journal of Cell Science. 117:1945-1953).
Immunohistochemistry Analysis: A representative lot detected Rab27B in rat parotid acinar cells (Imai, A., et al. (2004). Journal of Cell Science. 117:1945-1953).
Western Blotting Analysis: A representative lot detected Rab27B in rat parotid gland lysate (Imai, A., et al. (2004). Journal of Cell Science. 117:1945-1953).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Rab27B in flg-Rab27B (Imai, A., et al. (2004). Journal of Cell Science. 117:1945-1953).
Immunohistochemistry Analysis: A representative lot detected Rab27B in rat parotid acinar cells (Imai, A., et al. (2004). Journal of Cell Science. 117:1945-1953).
Quality
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Rab27B in 10 µg of mouse brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Rab27B in 10 µg of mouse brain tissue lysate.
Target description
~27 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physical form
Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
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