ABE1339

Anti-dimethyl-BAF155 Antibody (Arg1064, asymetrically di-methylated)

from rabbit, purified by affinity chromatography

• Synonyma: BRG1-associated factor 155
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Vlastnosti

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• technique(s): ChIP: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NP_003065
• UniProt accession no.: Q92922
• shipped in: dry ice
• target post-translational modification: dimethylation (Arg1064)
• Gene Information: human ... SMARCC1(6599)

Popis

General description

BAF155, also known as SWI/SNF complex subunit BRG1-associated factor 155, BAF155, SWI/SNF complex 155 kDa subunit, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1, and encoded by the gene name SMARCC1 and BAF155, is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). BAF155 has been implied to stimulate the ATPase activity of the catalytic subunit of the complex. BAF155 may also be involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. BAF155 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). BAF155 is expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas.

Immunogen

Epitope: C-terminus

linear peptide corresponding to the C-terminus of Human BAF155 protein dimethylated at Arg 1064.

Application

Research Category
Epigenetics & Nuclear Function

Apoptosis & Cancer

Research Sub Category
Chromatin Biology

This Anti-dimethyl-BAF155 antibody is validated for use in WB, IP, ChIP, IH for the detection of dimethyl-BAF155.

Western Blotting Analysis: A representative lot of this antibody detected methylated BAF155 in transfected MCF-7 cell lysate (Wang, L., et al. Cancer Cell 25, 21–36, January 13, 2014)
Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated methylated BAF155 from transfected SKOV3 cells (Wang, L., et al. Cancer Cell 25, 21–36, January 13, 2014)
ChIP: A representative lot of this antibody chromatin immunoprecipitated BAF155 to COL1A2 and GADD45A genes in wildtype MCF-7 cells (Wang, L., et al. Cancer Cell 25, 21–36, January 13, 2014)
Immunohistochemical Analysis: A representative lot of this antibody detected methylated BAF155 protein in human breast tumor cells (Wang, L., et al. Cancer Cell 25, 21–36, January 13, 2014)

Quality

Evaluated by Western Blotting on MCF-7 cell lysate

Western Blotting Analysis: A 1:1000 dilution of this antibody detected dimethyl-BAF155 protein in 10 µg of MCF-7 cell lysate

Target description

~ 160 kDa observed. The ~160 kDa band has been confirmed to be the target protein BAF155 by mass spectrometry

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide and 10% glycerol.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Bezpečnostní informace

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2