ABC80
Anti-phospho AMBRA1 Antibody (Ser52)
from rabbit, purified by affinity chromatography
Synonyma:
Activating molecule in BECN1-regulated autophagy protein 1, Ambra, Ambra-1
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Doporučené produkty
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
rat (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology)
technique(s)
inhibition assay: suitable (peptide)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer52)
Gene Information
human ... AMBRA1(55626)
General description
Activating molecule in BECN1-regulated autophagy protein 1 (AMBRA1) interacts with BECN1, likely forming a complex with BECN1 and PIK3C3. AMBRA1 is involved in autophagy by controlling protein turnover during neuronal development, and in regulating differentiation, survival and proliferation of normal cells.
Specificity
This antibody recognizes AMBRA1 phosphorylated at Ser52.
Immunogen
Epitope: Phosphorylated Ser52
KLH-conjugated linear peptide corresponding to human AMBRA1 phosphorylated at Ser52.
Application
Detect Activating molecule in BECN1-regulated autophagy protein 1 using this rabbit polyclonal antibody, Anti-phospho AMBRA1 Antibody (Ser52) validated for use in western blotting & Peptide Inhibition Assay.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
Western Blotting/Peptide Inhibition Analysis: 2 µg/mL from a representative lot detected phospho AMBRA1 (Ser52) in WB/PIA.
Western Blotting Analysis: A representative lot from an independent laboratory detected phospho AMBRA1 (Ser52) in HEK293 cell lysates trated without Torin1 or EBS and demonstrated a loss of signal in HEK293 cell lysates treated with Torin1 or EBS (Nazio, F., et al. (2013). Nat Cell Biol.15(4):406-416.).
Western Blotting Analysis: A representative lot from an independent laboratory detected phospho AMBRA1 (Ser52) in HEK293 cell lysates trated without Torin1 or EBS and demonstrated a loss of signal in HEK293 cell lysates treated with Torin1 or EBS (Nazio, F., et al. (2013). Nat Cell Biol.15(4):406-416.).
Quality
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected phospho AMBRA1 (Ser52) in 10 µg of HEK293 cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected phospho AMBRA1 (Ser52) in 10 µg of HEK293 cell lysate.
Target description
~150 kDa observed. Uniprot describes 6 isoforms from 83 kDa to 143 kDa This protein may be observed at higher molecular weights possibly due to hyperphosphorylation (Nazio, F., et al. (2013). Nat Cell Biol. [Epub ahead of print].).
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HEK293 cell lysate
HEK293 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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