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AB3532P

Sigma-Aldrich

Anti-Epithelial Sodium Channel-β Antibody

Chemicon®, from rabbit

Synonyma:

ENaC-beta

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

NCBI accession no.

NM_000336.2

UniProt accession no.

P51168

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... SCNN1B(6338)

Specificity

Recognizes rat Epithelial Sodium Channel beta (ENaC beta). The immunogen shows no significant sequence homology with other ENaC subunits.

Immunogen

A 20 amino acid peptide sequence near the N-terminus of rat ENaC beta. The immunogen sequence is 100% conserved in mouse, 94% in rabbit, 81% in human, 76% in ovine and 64% in xenopus.

Application

Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

Neuroinflammation & Pain
This Anti-Epithelial Sodium Channel-β Antibody is validated for use in ELISA, WB for the detection of Epithelial Sodium Channel-β.
Western blot: 1-10 μg/mL using ECL. 83-90kDa bands, membrane preparations are recommended (Masilamani et al., 1999).

ELISA: 0.5-1.0 mg/mL using 1 mg/mL control peptide to coat plate.

Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin in PBS containing 0.1% BSA.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

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EMBO molecular medicine, 10(10) (2018-08-30)
The shift of cytokine profile from anti- to pro-inflammatory is the most recognizable sign of labor, although the underlying mechanism remains elusive. Here, we report that the epithelial sodium channel (ENaC) is upregulated and activated in the uterus at labor
L R E Pedersen et al.
Acta physiologica (Oxford, England), 206(2), 142-149 (2012-06-12)
We wished to determine the effect of continuous β-receptor stimulation on alveolar fluid clearance and to elucidate the mechanisms behind this effect. Alveolar fluid clearance was measured in anaesthetized rats pretreated for 72 h with the β-agonist isoproterenol (200 μg kg(-1) h(-1) sc)
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American journal of physiology. Renal physiology, 315(3), F653-F664 (2018-05-24)
The chronic intrinsic diuretic and natriuretic tone of sodium-glucose cotransporter 2 (SGLT2) inhibitors is incompletely understood because their effect on body fluid volume (BFV) has not been fully evaluated and because they often increase food and fluid intake at the

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