AB2215
Anti-Interferon-β Antibody
serum, Chemicon®
Synonyma:
Anti-IFB, Anti-IFF, Anti-IFN-beta, Anti-IFNB
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Doporučené produkty
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
neutralization: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
mouse ... Ifnb1(15977)
Specificity
Reacts with mouse Interferon beta (IFNbeta). No reactivity is seen with IFNalpha or gamma
Immunogen
Mouse IgG, whole molecule.
Application
Cytopathic effect inhibition assay: 2 x 10E5 neutralization units per mL of antiserum. One neutralization unit is the amount of antiserum required to neutralize 1 unit of mouse IFN-beta. This material is prepared specifically for effective neutralization of mouse IFN-beta.
Western blot (1:500) The antibody reacts with a 19kDa band coresponding to Mu-IFN-beta. Protocol below:
1. Put protein (approx 50ng) in blocking solution for 30-60 min RT
2. Add Primary antibody (1:500) to blocking solution
3. Agitate membrane for 60 min, RT
4. Wash 3X for 5min each with PBS-Tween
5. Add secondary antibody (1:1000) after diluting in PBS-Tween
6. Gentle agitation for 60min.
7. Wash 3X for 5min each with PBS-Tween
8. Develop with reaction kit of choice
Immunoprecipitation
Immunohistochemistry
ELISA
Optimal working dilutions must be determined by end user.
Western blot (1:500) The antibody reacts with a 19kDa band coresponding to Mu-IFN-beta. Protocol below:
1. Put protein (approx 50ng) in blocking solution for 30-60 min RT
2. Add Primary antibody (1:500) to blocking solution
3. Agitate membrane for 60 min, RT
4. Wash 3X for 5min each with PBS-Tween
5. Add secondary antibody (1:1000) after diluting in PBS-Tween
6. Gentle agitation for 60min.
7. Wash 3X for 5min each with PBS-Tween
8. Develop with reaction kit of choice
Immunoprecipitation
Immunohistochemistry
ELISA
Optimal working dilutions must be determined by end user.
Detect Interferon-β using this Anti-Interferon-β Antibody validated for use in ELISA, IP, WB, IH, NEUT.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
Cytokines & Cytokine Receptors
Packaging
2x10(e4) units
Physical form
Rabbit polyclonal serum in buffer containing 0.3 M NaHCO3 and 200 mM NaCl.
Storage and Stability
Maintain frozen at -20°C or below for retention of full activity, for up to 12 months. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Osvědčení o analýze (COA)
Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.
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