70911
Origami B Competent Cell Set - Novagen
Escherichia coli, rod shaped
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O této položce
UNSPSC Code:
41106202
NACRES:
NA.81
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Dovolte nám, abychom vám pomohliNázev produktu
Origami B Competent Cell Set - Novagen,
biological source
Escherichia coli
manufacturer/tradename
Novagen®
storage condition
OK to freeze
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
shipped in
dry ice
storage temp.
−70°C
Quality Level
Související kategorie
Disclaimer
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
General description
Origami B host strains carry the same trxB/gor mutations as the original Origami strains, except that they are derived from a lacZY mutant of BL21. Thus the Origami B strains combine the desirable characteristics of BL21, Tuner and Origami hosts in one strain background. The trxB and gor mutations are selectable on kanamycin and tetracycline, respectively; therefore, this strain is recommended for use with pET plasmids carrying the ampicillin resistance marker bla.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
Genotypes:
Origami B
F-ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC gor522::Tn10 trxB (KanR, TetR)
Origami B(DE3)
F-ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB (KanR, TetR)
Origami B(DE3)pLysS
F-ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB pLysS (CamR, KanR, TetR)
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Origami B
F-ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC gor522::Tn10 trxB (KanR, TetR)
Origami B(DE3)
F-ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB (KanR, TetR)
Origami B(DE3)pLysS
F-ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB pLysS (CamR, KanR, TetR)
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Other Notes
•2 x 0.2 mlOrigami B Competent Cells
•2 x 0.2 mlOrigami B(DE3) Competent Cells
•2 x 0.2 mlOrigami B(DE3)pLysS Competent Cells
•4 x 2 mlSOC Medium
•2 ngTest Plasmid
•2 x 0.2 mlOrigami B(DE3) Competent Cells
•2 x 0.2 mlOrigami B(DE3)pLysS Competent Cells
•4 x 2 mlSOC Medium
•2 ngTest Plasmid
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Skladovací třída
10 - Combustible liquids
wgk
WGK 2
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