70663
Proteinase K, Lyophilized
Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA.
Synonyma:
Proteinase K from Tritirachium album, Endopeptidase K
Přihlásit pro zobrazení organizačních a smluvních cen.
Vybrat velikost
100 MG
7 740,00 Kč
500 MG
24 700,00 Kč
7 740,00 Kč
K dispozici k odeslání DNESpodrobné informace
O této položce
Číslo CAS:
UNSPSC Code:
12352202
NACRES:
NA.85
MDL number:
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Proteinase K, Lyophilized, Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA.
form
solid
mol wt
28.93 kDa
manufacturer/tradename
Novagen®
storage condition
OK to freeze
technique(s)
DNA extraction: suitable
shipped in
wet ice
storage temp.
2-8°C
Quality Level
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Tato položka | SRE0047 | SAE0151 | 1.24568 |
|---|---|---|---|
| technique(s) DNA extraction: suitable | technique(s) - | technique(s) - | technique(s) - |
| form solid | form lyophilized powder | form - | form solid |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in - |
| storage condition OK to freeze | storage condition - | storage condition - | storage condition - |
| mol wt 28.93 kDa | mol wt 28.93 kDa | mol wt 28.93 kDa | mol wt 28.93 kDa |
Application
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
Biochem/physiol Actions
Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.
Disclaimer
Toxicity: Harmful (C)
General description
Proteinase K is a highly active 28,904-Daserine protease isolated from the fungusTritirachium album. The enzyme exhibitsbroad cleavage specificity on native anddenatured proteins and is widely used in thepurification of DNA and RNA. Its activityis increased in the presence of denaturantssuch as SDS (1%) and elevated temperature(50-60°C). The recommended workingconcentration is 50-100 µg/ml for proteinremoval and enzyme inactivation, and up to2 mg/ml for tissue treatment. The Proteinase K,Lyophilized powder can be prepared as a20 mg/ml stock solution in water and storedin aliquots at -20°C. The enzyme is alsoavailable as a ready-to-use concentrated stocksolution (600 mAU/ml) that is convenientfor routine use in most applications. 1 mgof Proteinase K is the equivalent of 30 mAU(AU = Anson unit). The Novagen ProteinaseK products are free of detectable DNase andRNase
Other Notes
One AU (AU = Anson unit) is defined as the amount of enzyme that liberates 1.0 µmol (181 µg) of tyrosine from casein per minute at pH 7.5 at 37°C.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Skladovací třída
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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