654270

TNP-470

A cell-permeable Fumagillin analog that inhibits MetAP2, but not MetAP1, methionine aminopeptidase activity, without affecting the inhibitory activity of MetAP2 against HRI- catalyzed eIF-2α phosphorylation.

• Synonyma: TNP-470, AGM-1470, O-(Chloroacetyl-carbamoyl)fumagillol, AGM1470, MetAP-2 Inhibitor II, MetAP2 Inhibitor II, Methionine Aminopeptidase 2 Inhibitor II, TNP470
• Empirický vzorec (Hillův zápis): C₁₉H₂₈ClNO₆
• Číslo CAS: 129298-91-5
• Molekulová hmotnost: 401.88
• MDL number: MFCD00870281
• UNSPSC Code: 12352200
• NACRES: NA.77

Vlastnosti

• Quality Level: 200
• assay: ≥97% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white to light yellow
• solubility: DMSO: 100 mg/mL
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: ClCC(=O)NC(=O)O[C@H]1[C@H]([C@@H]([C@]3(OC3)CC1)[C@]2(O[C@@H]2CC=C(C)C)C)OC
• InChI: 1S/C19H28ClNO6/c1-11(2)5-6-13-18(3,27-13)16-15(24-4)12(7-8-19(16)10-25-19)26-17(23)21-14(22)9-20/h5,12-13,15-16H,6-10H2,1-4H3,(H,21,22,23)/t12-,13-,15-,16-,18+,19+/m1/s1
• InChI key: MSHZHSPISPJWHW-PVDLLORBSA-N

Popis

General description

A cell-permeable Fumagillin (Cat. No. 344845) analog that inhibits MetAP2, but not MetAP1, methionine aminopeptidase activity (IC₅₀ = 1 nM against human MetAP2) by covalently modifying MetAP2 active site histidine (aa 231 in human/mouse/rat; aa 174 in yeast) via its epoxide group, without affecting the inhibitory activity of MetAP2 against HRI- (heme-regulated inhibitor kinase) catalyzed eIF-2α phosphorylation. Cellular MetAP2 inhibition is particularly effective in inducing endothelial cytostasis (IC_{50 = 25 pM and 37 pM, respectively, against HUVEC and bovine BAEC proliferation by TNP-470), making TNP-470 a potent anti-angiogenesis agent in vivo (2 to 100 mg/kg in mice and rats; s.c.). Reported to be more potent and less toxic than Fumagillin.}

Warning

Toxicity: Irritant (B)

Other Notes

Zhang, Y., et al. 2000. Proc. Natl. Acad. Sci. USA97, 6427.
Sin, N., et al. Proc. Natl. Acad. Sci. USA94, 6099.
Yamaoka, M., et al. 1993. Cancer Res.53, 4262.
Kusaka, M., et al. 1991. Biochem. Biophys. Res. Commun.174, 1070.
Ingber, D., et al. 1990. Nature348, 555.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Bezpečnostní informace

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable