616387

N^α-Tosyl-Phe Chloromethyl Ketone

Irreversible inhibitor of chymotrypsin.

• Synonyma: N^α-Tosyl-Phe Chloromethyl Ketone, TPCK
• Empirický vzorec (Hillův zápis): C₁₇H₁₈ClNO₃S
• Číslo CAS: 402-71-1
• Molekulová hmotnost: 351.85
• MDL number: MFCD00000935
• UNSPSC Code: 12352200
• NACRES: NA.77

Vlastnosti

• Quality Level: 100
• assay: ≥98% (TLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• solubility: ethanol: 5 mg/mL; methanol: 5 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: [S](=O)(=O)(N[C@@H](Cc2ccccc2)C(=O)CCl)c1ccc(cc1)C
• InChI: 1S/C17H18ClNO3S/c1-13-7-9-15(10-8-13)23(21,22)19-16(17(20)12-18)11-14-5-3-2-4-6-14/h2-10,16,19H,11-12H2,1H3/t16-/m0/s1
• InChI key: MQUQNUAYKLCRME-INIZCTEOSA-N

Popis

General description

Irreversible inhibitor of chymotrypsin. Useful for inhibiting chymotrypsin activity in trypsin preparations. Inhibits apoptosis in thymocytes. Blocks the induction of nitric oxide synthase by γ-interferon and lipopolysaccharide (EC₅₀ = 20 µM). Suggested working concentration is 10-100 µM.

This product has been discontinued.


Irreversible inhibitor of chymotrypsin. Useful for inhibiting chymotrypsin activity in trypsin preparations. Inhibits apoptosis in thymocytes. Blocks the induction of nitric oxide synthase by γ-interferon and lipopolysaccharides (EC₅₀ = 20 M).

Biochem/physiol Actions

Cell permeable: no

EC50 = 20 µM in blocking the induction of nitric oxide synthase by γ-interferon and lipopolysaccharides.

Primary Target
chymotrypsin activity in preparations

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Irritant (B)

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 4 months at 4°C.

Other Notes

Fearnhead, H.O., et al. 1995. FEBS Lett. 357, 242.
Griscavage, J.M., et al. 1995. Biochem. Biophys. Res. Commun.215, 721.
Weaver, V.M., et al. 1993. Biochem. Cell Biol.71, 488.
Murakami, T., et al. 1991. Biochim. Biophys. Acta1079, 279.
Nierodzik, M.L., et al. 1991. J. Clin. Invest.87, 229.
Wilson, W.E., et al. 1989. J. Biol. Chem. 264, 17777.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Bezpečnostní informace

• pictograms: GHS05,GHS07
• signalword: Danger
• hcodes: H315,H318,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable