5.38339

CMLD-2

≥98% (HPLC), RNA stability modulator, solid

• Synonyma: HuR-ARE Interaction Inhibitor, CMLD-2, 5,7-dimethoxy-8-(1-(4-methoxyphenyl)-3-oxo-3-(pyrrolidin-1-yl)propyl)-4-phenyl-2H-chromen-2-one
• Empirický vzorec (Hillův zápis): C₃₁H₃₁NO₆
• Molekulová hmotnost: 513.58
• UNSPSC Code: 12352200
• NACRES: NA.77

Vlastnosti

• Název produktu: HuR-ARE Interaction Inhibitor, CMLD-2,
• Quality Level: 100
• assay: ≥98% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: white
• solubility: DMSO: 50 mg/mL
• storage temp.: 2-8°C

Popis

Biochem/physiol Actions

Cell permeable: yes

Primary Target
HuR

Reversible: yes

Target K_i: 350 nM for HuR binding

Disclaimer

Toxicity: Standard Handling (A)

General description

A cell-permeable, 2H-chromen-2-one based compound that competitively binds to Hu antigen R (HuR; K_i = 350 nM) and disrupts the interaction of HuR and adenine- and uridine-rich element (ARE) of target mRNAs. Displays dose-dependent binding to full-length HuR protein and to RRM1/2 fragment. Exhibits cytotoxicity towards tumor cells and reduces their viability (IC₅₀ = 28.9 and 18.2 µM for HCT-116 and MioPaCa2 cells, respectively). Has much reduced effect on normal WI-38 and CCD 841 CoN cells. Shown to induce cleavage of poly (ADP-ribose) polymerase and pro-caspase 3 (~ 50 µM) and reduce the half-life of Bcl-2, Msi1, and XIAP in HCT-116 cells (~20 µM) treated with actinomycin D (5 µg/ml).

Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.

Other Notes

Wu, X., et al. 2015. ACS Chem. Biol.10, 1476.

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20&deg:). Stock solutions are stable for up to months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Bezpečnostní informace

• Skladovací třída: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable