208721

ALLM

Cell permeable inhibitor of calpain I (Ki = 120 nM), calpain II (Ki = 230 nM), cathepsin B (K_i = 100 nM), and cathepsin L (K_i = 600 pM).

• Synonyma: ALLM, Calpain Inhibitor II
• Empirický vzorec (Hillův zápis): C₁₉H₃₅N₃O₄S
• Číslo CAS: 110115-07-6
• Molekulová hmotnost: 401.56
• UNSPSC Code: 12352200
• NACRES: NA.77

Vlastnosti

• Quality Level: 100
• assay: >95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: off-white
• solubility: DMSO: 5 mg/mL; ethanol: 5 mg/mL; methanol: 5 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C
• InChI: 1S/C19H35N3O4S/c1-12(2)9-16(20-14(5)24)19(26)22-17(10-13(3)4)18(25)21-15(11-23)7-8-27-6/h11-13,15-17H,7-10H2,1-6H3,(H,20,24)(H,21,25)(H,22,26)/t15-,16-,17-/m0/s1
• InChI key: RJWLAIMXRBDUMH-ULQDDVLXSA-N

Popis

General description

A cell-permeable inhibitor of calpain I (K_i = 120 nM), calpain II (K_i = 230 nM), cathepsin B (K_i = 100 nM), and cathepsin L (K_i = 0.6 nM). Inhibits activation-induced programmed cell death and restores defective immune responses in HIV⁺ donors. Also prevents nitric oxide produced by activated macrophages by interfering with transcription of the inducible nitric oxide synthase gene.

Cell permeable inhibitor of calpain I (K_i = 120 nM), calpain II (K_i = 230 nM), cathepsin B (K_i = 100 nM), and cathepsin L (K_i = 600 pM). Inhibits activation-induced programmed cell death and restores defective immune responses in HIV⁺ donors. Blocks nitric oxide production by activated macrophages by interfering with transcription of the inducible nitric oxide synthase gene. A weak inhibitor of proteasome.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
calpain-1

Product does not compete with ATP.

Reversible: no

Target K_i: 120 nM, 230 nM, 100 nM, and 600 pM, against calpain I, calpain II, cathepsin B, and cathepsin L, respectively

Warning

Toxicity: Standard Handling (A)

Sequence

N-Acetyl-Leu-Leu-Met

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Ravid, T., et al. 2000. J. Biol. Chem.275, 35840.
Griscavage, J.M., et al. 1995. Biochem. Biophys. Res. Commun.215, 721.
Sarin, A., et al. 1994. J. Immunol.153, 862.
Sarin, A., et al. 1993. J. Exp. Med. 178, 1693.
Banik, N.L., et al. 1992. Neurochem. Res.17, 797.
Koohmaraie, M. 1992. Biochemie74, 239.
Pinter, M., et al. 1992. Biochemistry31, 8201.
Shenoy, A.M., and Brahmi, Z. 1991. Cell. Immunol.138, 24.
Sasaki, T., et al. 1990. J. Enzyme Inhib.3, 195.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Bezpečnostní informace

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable