17-327

H2A.X Phosphorylation Assay Kit (Chemiluminescence Detection)

Name: H2A.X Phosphorylation Assay Kit (Chemiluminescence Detection)
Brand: MM
Price: 24300 CZK

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NACRES:

NA.54

UNSPSC Code:

12161503

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packaging

kit of 192 assay(s)

manufacturer/tradename

Upstate^®

technique(s)

ELISA: suitable

detection method

chemiluminescent

Quality Level

100

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Tato položka	17-344	05-636-I	05-636-AF488
Sigma-Aldrich 17-327 H2A.X Phosphorylation Assay Kit (Chemiluminescence Detection)	Sigma-Aldrich 17-344 H2A.X Phosphorylation Assay Kit (Flow Cytometry)	Sigma-Aldrich 05-636-I Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301	Sigma-Aldrich 05-636-AF488 Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor^™ 488 Conjugate Antibody
technique(s) ELISA: suitable	technique(s) activity assay: suitable, flow cytometry: suitable	technique(s) ChIP: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable	technique(s) immunocytochemistry: suitable
packaging kit of 192 assay(s)	packaging -	packaging -	packaging -
manufacturer/tradename Upstate^®	manufacturer/tradename Upstate^®	manufacturer/tradename -	manufacturer/tradename -
detection method chemiluminescent	detection method fluorometric	detection method -	detection method -
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100

General description

Phosphorylation of the histone variant H2A.X is a rapid and sensitive response to double strand DNA breaks. The H2A.X Phosphorylation Assay Kit (Chemiluminescence Detection) is a cell based assay formatted for microplate-based detection of levels of phosphorylated histone H2A.X.

Application

Analysis Note

The H2A.X Phosphorylation, Chemiluminescence Detection Assay Kit is a cell-based ELISA formatted for chemiluminescent detection of relative levels of phosphorylated H2A.X in microplate cells cultures. Adherent cells are cultured in microplates, treated with agents that induce DNA damage/H2A.X phosphorylation, and are then fixed and permeabilized. Histone H2A.X phosphorylated at serine 139 is detected by Anti-phospho-H2A.X (Ser139), clone JBW301 and an anti-mouse-HRP (horseradish peroxidase) conjugate. The chemiluminescent HRP substrate LumiGLO is then added, and signal is measured in a microplate luminometer

Other Notes

Anti-phospho-Histone H2A.X (Ser139), clone JBW301 (Cat.# 05-636)
TBS, 20X (Cat.# 20-190)
20% Tween-20 (v/v)
10% BSA in TBS (Blocking Buffer)
LumiGLO Substrate Reagent A
LumiGLO Substrate Reagent B
Goat Anti-Mouse IgG, HRP conjugate

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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