17-283

Rac1 Activation Assay Kit

Name: Rac1 Activation Assay Kit
Brand: MM
Price: 26100 CZK
Availability: InStock

Non-radioactive Rac1 Activation Assay Kit can be used to precipitate Rac1-GTP from cell lysates & detection by a Rac1 specific monoclonal antibody.

Synonyma:

Activation Assay Kit, Rac1 Activity Kit, Rac1 Assay Kit

Slide 1 of 1

Přihlásit pro zobrazení organizačních a smluvních cen.

Vybrat velikost

1 KIT

26 100,00 Kč

Očekávané datum odeslání29. prosince 2025podrobné informace

O této položce

UNSPSC Code:

12161503

eCl@ss:

32161000

NACRES:

NA.32

Hlavní dokumenty

Zobrazit celou dokumentaci

Přeskočit na

Technický servis

Potřebujete pomoc? Náš tým zkušených odborníků je vám k dispozici.

Dovolte nám, abychom vám pomohli

Quality Level

100

species reactivity

human, rat, mouse

manufacturer/tradename

Upstate^®

technique(s)

activity assay: suitable
affinity binding assay: suitable

NCBI accession no.

NM_006908.3
NM_018890.2
NM_198829.1

UniProt accession no.

P63000

shipped in

dry ice

Gene Information

human ... RAC1(5879)

Porovnat podobné položky

Zobrazit úplné porovnání

Zobrazit rozdíly

1 of 4

Tato položka	17-441	14-325	09-271
Sigma-Aldrich 17-283 Rac1 Activation Assay Kit	Sigma-Aldrich 17-441 Rac1/Cdc42 Activation Assay Kit	Sigma-Aldrich 14-325 Rac/cdc42 Assay Reagent (PAK1 PBD, agarose), 300 µg	Sigma-Aldrich 09-271 Anti-Rac1b Antibody
technique(s) activity assay: suitable, affinity binding assay: suitable	technique(s) activity assay: suitable, affinity binding assay: suitable (G-protein)	technique(s) affinity binding assay: suitable	technique(s) immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable
species reactivity human, rat, mouse	species reactivity human, mouse, rat	species reactivity -	species reactivity mouse, human
manufacturer/tradename Upstate^®	manufacturer/tradename Upstate^®	manufacturer/tradename Upstate^®	manufacturer/tradename Upstate^®
NCBI accession no. NM_006908.3	NCBI accession no. NM_006908.3, NM_018890.2, NM_198829.1	NCBI accession no. NM_001039802.1, NM_001791.3, NM_044472.2	NCBI accession no. NP_061485.1
UniProt accession no. P63000	UniProt accession no. P63000	UniProt accession no. P60953	UniProt accession no. P63000
shipped in dry ice	shipped in wet ice	shipped in dry ice	shipped in wet ice

General description

Non-radioactive Rac1 Activation Assay Kit can be used to precipitate Ras-related C3 botulinum toxin substrate 1 (Rac1)-GTP from cell lysates and detected by a Rac1 specific monoclonal antibody. Rac1 belongs to the Ras superfamily of small guanosine triphosphatases (GTPases). It is expressed in two major forms such as an inactive GDP-bound form and an active GTP-bound form. Rac1 is involved in influencing several physiological conditions by interacting with effectors that activate several signaling cascades.[1] The RAC1 gene is located on the human chromosome at 7p22.1.[2]

Application

Rac1 Activation Assay Kit has been used in the detection of active Ras-related C3 botulinum toxin substrate 1 (Rac1) in brain homogenates of mice and humans by immunoblotting.[3]

Analysis Note

Routinely evaluated by precipitating Rac-GTP from 3T3 cell lysates that had been loaded with GTPγS. The precipitated Rac-GTP was detected by immunoblot analysis using anti-Rac.

Other Notes

Anti-Rac1, clone 23A8 (Cat.# 05-389)

Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)

100X GTPγS, 10mM (Cat.# 20-176)

100X GDP, 100mM (Cat.# 20-177)

Rac/cdc42 Assay Reagent (PAK1 PBD, agarose) (Cat.# 14-325)

Replaces: SGT425

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

GHS05,GHS09

signalword

Danger

hcodes

H318,H410

pcodes

P273 - P280 - P305 + P351 + P338 - P391 - P501

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1

Skladovací třída

10 - Combustible liquids

Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

Již tento produkt vlastníte?

Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.

Navštívit knihovnu dokumentů

The role of mechanical stretching in the activation and localization of adhesion proteins and related intracellular molecules.

Boccafoschi, F, et al.

Journal of Cellular Biochemistry, 112, 1403-1409 (2011)

rs12977 of RAC1 is associated with the progress of gastric cancer in Iranian population

Javani A, et al.

Gene Reports, 23, 101069-101069 (2021)

The survival kinase Mirk/dyrk1B is activated through Rac1-MKK3 signaling.

Kideok Jin, Seunghwan Lim, Stephen E Mercer, Eileen Friedman

The Journal of Biological Chemistry null

Anti-arrhythmic surgery for atrioventricular junctional and atrioventricular re-entrant tachycardia--a report of six cases.

Koo, C C, et al.

Annals of the Academy of Medicine, Singapore, 19, 67-72 (1990)

Distinct sites within the vascular cell adhesion molecule-1 (VCAM-1) cytoplasmic domain regulate VCAM-1 activation of calcium fluxes versus Rac1 during leukocyte transendothelial migration.

Michelle E Marchese et al.

Biochemistry, 51(41), 8235-8246 (2012-09-14)

Vascular adhesion molecules regulate the migration of leukocytes from the blood into tissue during inflammation. Binding of leukocytes to vascular cell adhesion molecule-1 (VCAM-1) activates signals in endothelial cells, including Rac1 and calcium fluxes. These VCAM-1 signals are required for

Zobrazit všechny související dokumenty

Související obsah

Hallmarks of Aging

"Aging: getting older, exhibiting the signs of age, the decline in the physical (and mental) well-being over time, leading to death. Since the beginning of time, man has been obsessed with trying to slow down, stop, or even reverse the signs of aging. Many have gone as far as experimenting with nutritional regimens, eccentric exercises, fantastic rituals, and naturally occurring or synthetic wonder-elements to evade the signs of normal aging. Biologically speaking, what is aging? And what does the latest research tell us about the possibility of discovering the elusive “fountain of youth”? Many advances in our understanding of aging have come from systematic scientific research, and perhaps it holds the key to immortality. Scientifically, aging can be defined as a systems-wide decline in organismal function that occurs over time. This decline occurs as a result of numerous events in the organism, and these events can be classified into nine “hallmarks” of aging, as proposed by López-Otin et al. (2013). Several of the pathologies associated with aging are a direct result of these events going to extremes and may also involve aberrant activation of proliferation signals or hyperactivity. The hallmarks of aging have been defined based on their fulfillment of specific aging related criteria, such as manifestation during normal aging, acceleration of aging if experimentally induced or aggravated, and retardation of aging if prevented or blocked, resulting in increased lifespan. The nine hallmarks of aging are genomic instability, telomere attrition, epigenetic alterations, loss of proteostasis, deregulated nutrient sensing, mitochondrial dysfunction, cellular senescence, stem cell exhaustion, and altered intercellular communication. The biological processes underlying aging are complex. By understanding the hallmarks in greater detail, we can get closer to developing intervention strategies that can make the aging process less of a decline, and more of a recline."

PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

Questions

Reviews

No rating value

Active Filters

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

Obraťte se na technický servis