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Key Documents

14-383

Sigma-Aldrich

Rho Assay Reagent (Rhotekin RBD, agarose), 650 µg

Specifically binds to & precipitates GTP-Rho, but not GDP-Rho from cell lysates. For use in Affinity Binding Assays.

Synonyma:

Rho Assay Reagent, Rho Detection Reagent, Rhotekin RBD Assay Reagent

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen


About This Item

UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.84

biological source

mouse

Quality Level

manufacturer/tradename

Upstate®

technique(s)

affinity binding assay: suitable

UniProt accession no.

shipped in

dry ice

Gene Information

mouse ... RTKN(20166)

General description

GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E.coli and bound to glutathione-agarose. Specifically binds to and precipitates GTP-Rho, but not GDP-Rho from cell lysates.
Product Source: Expressed in E. coli

Quality

Routinely evaluated by precipitating GTP-Rho from 3T3/A31 lysates, subsequent detection by immunoblot analysis using Anti-RhoA, clone 55 (05-778).

Physical form

650 µg of recombinant protein in 1 mL glutathione-agarose slurry containing 45mM TRIS, pH 7.5, 0.45% Triton X-100, 135mM NaCl, 4.5mM MgCl2, 0.9mM dithiothreitol, 0.09mM PMSF, 0.9 µg/mL aprotinin, 0.9 µg/mL leupeptin, and 10% glycerol.

Storage and Stability

Stable for 6 months at -70°C from date of receipt.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

hcodes

Hazard Classifications

Aquatic Chronic 3

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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