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Merck

14-173-M

MAP Kinase 2/Erk2 Protein, active, mouse, 10 µg

Active, N-terminal GST-tagged, recombinant MAP Kinase 2, expressed in E. coli cells, activated using MEK1 (Catalog #14-429). For use in Kinase Assays.

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Vybrat velikost

10 μG

14 400,00 Kč

250 μG

93 000,00 Kč

14 400,00 Kč

Očekávané datum odeslání20. února 2026podrobné informace

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O této položce

UNSPSC Code:
12352202
NACRES:
NA.41
eCl@ss:
32160405
Biological source:
mouse
Recombinant:
expressed in E. coli

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biological source

mouse

recombinant

expressed in E. coli

mol wt

Mw 67.8 kDa

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

NM_011949.3

UniProt accession no.

P63085

Quality Level

100

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Tato položka
14-550-M14-439-M14-198
MAP Kinase 2/Erk2 Protein, active, mouse, 10 µg Active, N-terminal GST-tagged, recombinant MAP Kinase 2, expressed in E. coli cells, activated using MEK1 (Catalog #14-429). For use in Kinase Assays.

Sigma-Aldrich

14-173-M

MAP Kinase 2/Erk2 Protein, active, mouse, 10 µg

MAP Kinase 2/Erk2 Protein, active, Human, 10 µg Active, N-terminal GST tagged, recombinant, full-length, human MAP Kinase 2/Erk2. Activated with MEK1 (Cat. # 14-429). For use in Kinase Assays.

Sigma-Aldrich

14-550-M

MAP Kinase 2/Erk2 Protein, active, Human, 10 µg

MAP Kinase 1/Erk1 Protein, active, 10 µg Active, recombinant, full-length, human MAP Kinase 1/Erk1 containing an N-terminal GST tag, for use in Kinase Assays.

Sigma-Aldrich

14-439-M

MAP Kinase 1/Erk1 Protein, active, 10 µg

MAP Kinase 2/Erk2 Protein, inactive, Mouse, 50 g Unactive, recombinant full-length mouse p42 MAP Kinase 2/Erk2 fused with GST at the N terminus, for use in Kinase Assays.

Sigma-Aldrich

14-198

MAP Kinase 2/Erk2 Protein, inactive, Mouse, 50 g

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

biological source

mouse

biological source

human

biological source

human

biological source

mouse

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

UniProt accession no.

P63085

UniProt accession no.

P28482

UniProt accession no.

P28482

UniProt accession no.

P63085

mol wt

Mw 67.8 kDa

mol wt

Mw 67.8 kDa

mol wt

Mw 69.9 kDa

mol wt

Mw 67.8 kDa

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

General description

N-terminal GST-tagged, recombinant MAP Kinase 2, expressed in E. coli cells; activated using MEK1 (Catalog #14-429).
Product Source: recombinant, mouse, full-length MAP Kinase 2

Biochem/physiol Actions

Protein Target: MAPK2
Target Sub-Family: CMGC

Packaging

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-173M when ordering the 250 µg size.

Physical form

50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 50% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
Glutathione agarose; Ni2+/NTA agarose

Preparation Note

Store in undiluted aliquots at -20°C for up to one year from the date of receipt. Avoid repeatede freeze-thaw cycles.

Analysis Note

Routinely evaluated by phosphorylation myelin basic protein

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Skladovací třída

10 - Combustible liquids

wgk

WGK 2

Osvědčení o analýze (COA)

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Tyrosyl phosphorylation and activation of the myelin basic protein kinase p44mpk during sea star oocyte maturation.
Sanghera, J S, et al.
Biochimica et Biophysica Acta, 1095, 153-160 (1991)
Purification and characterization of a maturation-activated myelin basic protein kinase from sea star oocytes.
Sanghera, J S, et al.
The Journal of Biological Chemistry, 265, 52-57 (1990)
X Yang et al.
The Journal of biological chemistry, 273(45), 29879-29887 (1998-10-29)
The human immunodeficiency virus type 1 (HIV-1) Vif protein plays a critical role in virus replication and infectivity. Here we show that Vif is phosphorylated and regulated by p44/42 mitogen-activated protein kinase (MAPK). Vif phosphorylation by MAPK was demonstrated in
Identification of insulin-stimulated protein kinase-1 as the rabbit equivalent of rskmo-2. Identification of two threonines phosphorylated during activation by mitogen-activated protein kinase.
Sutherland, C, et al.
European Journal of Biochemistry, 212, 581-588 (1993)
C Zhang et al.
The Journal of biological chemistry, 272(20), 13397-13402 (1997-05-16)
Aggregation of the high affinity IgE receptor (FcepsilonRI) in a mast cell line resulted in activation of the p42 and the stress-activated p38 mitogen-activated protein (MAP) kinases. Selective inhibition of these respective kinases with PD 098059 and SB 203580 indicated
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