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Merck
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Key Documents

05-531

Sigma-Aldrich

Anti-His Tag Antibody

clone 4D11, mouse monoclonal, Upstate

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43

product name

Anti-Histidine Tagged Antibody, clone 4D11, clone 4D11, Upstate®, from mouse

biological source

mouse

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

4D11, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG2aκ

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... HRG(3273)

Specificity

Recognizes tag sequence HHHHHHGS on either the N- or C-terminus of recombinant proteins; GS amino acids are required for recognition of 6His tag

Immunogen

N-terminal His-tagged and truncated TRAP protein corresponding to amino acids 26-248 from P. falciparum expressed in E coli.

Application

This Anti-Histidine Tagged Antibody (anti His antibody) clone 4D11 is validated for use in IC, WB for the detection of Histidine Tagged.

Quality

routinely evaluated by immunoblot on recombinant N-terminal and C-terminal His-tagged proteins

Target description

The apparent molecular weight is related to the tagged protein.

Physical form

Format: Purified
Protein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.014M phosphate buffer, pH 7.6, 0.175M NaCl, and 0.07% sodium azide.

Analysis Note

Control
Cells expressing the His-tag

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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