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Merck

U1500

Sigma-Aldrich

Urease from Canavalia ensiformis (Jack bean)

Type III, powder, 15,000-50,000 units/g solid

Sinónimos:

Jack bean urease, Urea amidohydrolase

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About This Item

Número de CAS:
Comisión internacional de enzimas:
EC Number:
MDL number:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

biological source

Canavalia ensiformis

Quality Level

type

Type III

form

powder

specific activity

15,000-50,000 units/g solid

mol wt

~544620 Da

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

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General description

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Urease is a cytosolic enzyme that catalyzes the hydrolysis of urea to ammonia and carbon dioxide, with initial conversion to carbamate, and the carbamate breaks down further, on hydrolysis, into ammonia and to carbonic acid / carbonate. Urease is a metalloenzyme that contains nickel as a co-factor. Urease occurs naturally in plants, fungi, and certain bacteria. James B. Sumner first isolated urease from jack bean in 1926.

Biochem/physiol Actions

Urease from Canavalia ensiformis functions as an insecticide by the release of a toxic small peptide cleaved by proteases found in the gut of insects.

Unit Definition

1 U setzt 1.0 μmol NH3 aus Harnstoff pro Minute bei pH 7.0 und 25 °C frei. Es ist äquivalent zu 1.0 I.U. oder 0.054 Sumner Einheiten (1.0 mg Ammoniak Stickstoff in 5 Minuten bei pH 7.0 und 20 °C)

pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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