SAE0152

Lisozima from chicken egg white

free of DNA contaminants, suitable for Microbiome research, lyophilized powder, protein ≥90%, ≥40,000 units/mg protein

• Sinónimos: Mucopéptido N-acetilmuramoilhidrolasa, Muramidasa
• Número de CAS: 12650-88-3
• Comisión internacional de enzimas: 3.2.1.17 (BRENDA, IUBMB)
• MDL number: MFCD00131557
• UNSPSC Code: 12352204
• NACRES: NA.77

Propiedades

• biological source: chicken egg white
• Quality Level: 100
• form: powder
• specific activity: ≥40000 units/mg protein
• mol wt: single-chain 14.3 kDa
• feature: DNA free
• technique(s): cell based assay: suitable
• suitability: suitable for cell lysis
• application(s): cell analysis
• shipped in: wet ice
• storage temp.: −20°C

Descripción

General description

Purified Lysozyme DNA free SAE0152 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.

Lysozyme is a single chain polypeptide of 129 amino acids cross-linked with four disulfide bridges.1 It hydrolyzes b(1à 4) linkages between N-acetylmuraminic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin.2,3,4 The enzyme is often used for lysing bacterial cells by hydrolyzing the peptidoglycan present in the cell walls. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan.
The enzyme is active over a broad pH range (6.0-9.0).

Lysozyme activity: ≥40,000 units/mg protein.

For isolation of nucleic acids, lysozyme has been used in the lysis peptidoglycan layer of bacterial cell walls.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminates are essential. This purified lysozyme preparation (SAE0152) is purified from chicken egg white, crystallized three times, dialyzed, and supplied as a lyophilized powder. Protein content by UV absorbance is ≥ 90% with the remainder (∼10%) being buffer salts such as sodium acetate and sodium chloride, and undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.

Application

• Lysozyme is used for the extraction of genomic DNA from bacterial cells.
• Lysozyme is used as an external standard for MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass analysis.
• Lysonzyme is used to prepare spheroplasts.

La enzima rompe las paredes celulares de las bacterias; se utiliza para preparar esferoplastos.

Biochem/physiol Actions

La lisozima hidroliza los enlaces β(1→4) entre los restos del ácido N-acetilmurámico y los restos de N-acetil-D-glucosamina en el peptidoglucano y entre los residuos de N-acetil-D-glucosamina en la quitodextrina. Las células grampositivas son bastante sensibles a esta hidrólisis, ya que sus paredes celulares tienen una elevada proporción de peptidoglucanos. Las bacterias gramnegativas son menos sensibles debido a la presencia de una membrana externa y a una menor proporción de peptidoglucanos. Sin embargo, estas células pueden ser hidrolizadas en presencia de EDTA que forma complejos con los iones metálicos en la membrana bacteriana externa.

La enzima es activa en un amplio intervalo de pH (de 6,0 a 9,0). A pH 6,2, se observa actividad máxima en un intervalo de fuerzas iónicas más amplio (de 0,02 a 0,100 M) que a pH 9,2 (de 0,01 a 0,06 M).

Features and Benefits

Purified Lysozyme free SAE0152 undergoes strict quality control testing to ensure it will be Free of DNA contaminants, suitable for Microbiome research.

The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminates are essential.

Unit Definition

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

Información de seguridad

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable