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Key Documents

S9549

Sigma-Aldrich

Superoxide dismutase–polyethylene glycol from bovine erythrocytes

lyophilized powder, ≥1,350 units/mg protein

Sinónimos:

PEG-SOD

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About This Item

MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

Quality Level

200
400

specific activity

≥1,350 units/mg protein

mol wt

5000 Da

composition

Protein, ≥20% biuret

extent of labeling

≥10 mol PEG per mol protein

storage temp.

−20°C

General description

Superoxide dismutase from bovine erythrocytes is a copper and zinc superoxide dismutase. Copper and zinc ions are coordinated by active site histidine residues. It has β-barrel structure.

Application

Superoxide dismutase-polyethylene glycol from bovine erythrocytes has been used:
  • in the incubation of lung slices for measurement of dihydroethidium (DHE) fluorescence in cultured bovine pulmonary artery endothelial cells
  • as supplement in T cell culture to investigate its effect on cytokine production
  • in the preincubation of the carotid artery sections prior to DHE treatment

Biochem/physiol Actions

Superoxide dismutase-polyethylene glycol (SOD-PEG) is effective than SOD in scavenging free radicals in injuries. It reduces oxidative damage in ovarian follicles cell culture and during ovarian tissue transplantation. SOD-PEG is also effective in scavenging superoxide in mitochondrial abnormalities.

Packaging

Package size based on protein content

Other Notes

Superoxide dismutase from bovine erythrocytes coupled to methoxy-polyethylene glycol, M.W. 5,000, through secondary amine linkage.

Physical form

Lyophilized powder containing PEG plus 5% citrate buffer salts

Preparation Note

Enzyme derived

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

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Roles of zinc and copper in modulating the oxidative refolding of bovine copper, zinc superoxide dismutase
Li HT, et al.
Acta biochimica et biophysica Sinica, 42(3), 183-194 (2010)
The beneficial effects of polyethylene glycol-superoxide dismutase on ovarian tissue culture and transplantation
Kim EJ, et al.
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Yi X, Yuan D, Farr SA, et al.
Journal of Controlled Release : Official Journal of the Controlled Release Society, 191, 34-46 (2014)
Polyethylene glycol-superoxide dismutase prevents endotoxin-induced cardiac dysfunction
Supinski GS and Callahan LA
American Journal of Respiratory and Critical Care Medicine, 173(11), 1240-1247 (2006)

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