R8000
D-Ribulose 1,5-Diphosphate Carboxylase from spinach
partially purified powder, 0.01-0.1 unit/mg solid
Sinónimos:
3-Phospho-D-glycerate carboxy-lyase(dimerizing), Rubisco
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About This Item
Número de CAS:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
Productos recomendados
origen biológico
spinach
Nivel de calidad
Formulario
partially purified powder
actividad específica
0.01-0.1 unit/mg solid
mol peso
557 kDa
temp. de almacenamiento
−20°C
Descripción general
D-Ribulose 1,5-Diphosphate Carboxylase (RUBISCO) amounts to 50% of the total spinach leaves associated soluble protein.
Exists as a 557 kDa hexadecamer composed of eight heavy chains each with a molecular weight of approximately 56 kDa and eight light chains of molecular weight 14 kDa. Each molecule contains one magnesium ion.
pH optimum: ~7.9.
KM for CO2: ~0.45 mM.
Ribulose diphosphate becomes inhibitory at concentrations exceeding 0.7 mM. Orthophosphate and ammonium sulfate are competitive inhibitors. 3-Phosphoglycerate is a noncompetitive inhibitor.
pH optimum: ~7.9.
KM for CO2: ~0.45 mM.
Ribulose diphosphate becomes inhibitory at concentrations exceeding 0.7 mM. Orthophosphate and ammonium sulfate are competitive inhibitors. 3-Phosphoglycerate is a noncompetitive inhibitor.
Aplicación
D-Ribulose 1,5-Diphosphate Carboxylase from spinach has been used:
- as a test protein in pepsin digestion studies
- as an innocuous or non-hazardous protein sample to test its effect on human intestinal epithelial cell lines
- in isothermal titration calorimetry (ITC), and radiolabeled binding assays with abscisic acid
Acciones bioquímicas o fisiológicas
D-Ribulose 1,5-Diphosphate Carboxylase (RUBISCO) depends on Rubisco activase and chaperones for activation. It participates in plant photorespiration events by catalyzing the carboxylation and oxygenation of ribulose-1,5-bisphosphate. Abscisic acid inhibits the carboxylation activity of Rubisco.
Definición de unidad
One unit will convert 1.0 μmole of D-RuDP and CO2 to 2.0 μmoles of D-3-phosphoglycerate per min at pH 7.8 at 25°C.
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Resp. Sens. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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