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L4130

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O111:B4

purified by trichloroacetic acid extraction

Sinónimos:

LPS

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About This Item

EC Number:
297-473-0
MDL number:
MFCD00164401
UNSPSC Code:
12352201
NACRES:
NA.25
En este momento no podemos mostrarle ni los precios ni la disponibilidad

biological source

Escherichia coli (O111:B4)

Quality Level

200

form

lyophilized powder

purified by

trichloroacetic acid extraction

impurities

1-10% Protein (Lowry)

color

white to light yellow

solubility

water: soluble

shipped in

ambient

storage temp.

2-8°C

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General description

This product is TCA extracted from E. coli serotype O111:B4. The source strain is from a private collection. This LPS serotype has been used to stimulate B-cells and induce NOS in human hepatocytes.

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

related product

Referencia del producto
Descripción
Precios

L4391

Lipopolysaccharides from Escherichia coli O111:B4, γ-irradiated, BioXtra, suitable for cell culture

L3012

Lipopolysaccharides from Escherichia coli O111:B4, purified by gel-filtration chromatography

L4130

Lipopolysaccharides from Escherichia coli O111:B4, purified by trichloroacetic acid extraction

L3024

Lipopolysaccharides from Escherichia coli O111:B4, purified by ion-exchange chromatography, TLR ligand tested

L2630

Lipopolysaccharides from Escherichia coli O111:B4, purified by phenol extraction

L3023

Lipopolysaccharides from Escherichia coli O111:B4, Detoxified

F3665

Lipopolysaccharides from Escherichia coli O111:B4, FITC conjugate

L5293

Lipopolysaccharides from Escherichia coli O111:B4, Ready Made solution, 1 mg/mL

pictograms

Skull and crossbones
GHS06

signalword

Danger

hcodes

H300

Hazard Classifications

Acute Tox. 2 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000375986
0000375987
0000294863
0000294863
0000375987

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Visite la Librería de documentos

Marie Hilderman et al.
Clinical kidney journal, 8(5), 599-605 (2015-09-29)
The cholinergic anti-inflammatory pathway (CAP) modulates inflammatory responses through the vagus nerve and the α-7-nicotinic acetylcholine receptor (α7nAChR) on macrophages and immune cells. Sympathetic/parasympathetic imbalance and chronic inflammation are both linked to poor outcome in dialysis patients. The aim of
Lijia Chang et al.
The international journal of neuropsychopharmacology, 21(10), 932-937 (2018-06-13)
Although previous reports suggest sex-specific differences in the antidepressant actions of (R,S)-ketamine, these differences in the antidepressant actions of (R)-ketamine, which is more potent than (S)-ketamine, are unknown. Saline or (R)-ketamine was administered 23 hours post lipopolysaccharide administration to adult
Wei Yao et al.
European journal of pharmacology, 793, 21-27 (2016-11-07)
The Nuclear factor (erythroid 2-derived)-like 2 (Nrf2) plays a key role in inflammation which is implicated in the pathophysiology of depression. The Nrf2 activators have antidepressant effects in animal models of depression. The present study was undertaken to examine whether
Tobias Hilbert et al.
PloS one, 8(5), e65024-e65024 (2013-06-01)
IFNA1 (interferon alpha) is a key cytokine regulating the activity of numerous immune cells. Plasmacytoid dendritic cells (pDCs) as natural interferon-producing cells play critical roles as sensors of pathogens and link innate to adaptive immunity. CpG motifs within DNA sequences
Jun-Yan Liu et al.
Biochemical pharmacology, 79(6), 880-887 (2009-11-10)
Inflammation is a multi-staged process whose expansive phase is thought to be driven by acutely released arachidonic acid (AA) and its metabolites. Inhibition of cyclooxygenase (COX), lipoxygenase (LOX), or soluble epoxide hydrolase (sEH) is known to be anti-inflammatory. Inhibition of
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Questions

  1. Hi, The LPS (L4130) product data sheet does not include at what temperature it should be stored after dissolving with water or PBS. Can you help me with this issue, please?

    1 answer

    1. Please see the following link describing our LPS products: https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides

      Solutions at 1 mg/mL in buffer or culture medium are stable for approximately one month at 2-8 °C. For long term storage, frozen aliquots are stable up to 2 years. Avoid repeated freeze/thaw cycles. Solutions should be stored in silanized containers as LPS can bind to plastics and certain types of glass, particularly at concentrations of <0.1 mg/mL. If the LPS concentration is >1 mg/mL, adsorption to the sides of the vial is negligible. If glass containers are used, solutions should be vortexed for at least 30 minutes to redissolve any adsorbed product.

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