GE17-5255-01

HisTrap^™ Fast Flow

Cytiva 17-5255-01, pack of 5 × 5 mL

• Sinónimos: HisTrap FF, His-tagged protein purification column, ready-to-use column
• Código UNSPSC: 23151816
• NACRES: NA.56

Propiedades

• envase: pack of 5 × 5 mL
• fabricante / nombre comercial: Cytiva 17-5255-01
• Parámetros: <4 mL/min flow rate; 42 psi
• lecho tamaño: 7 mm × 25 mm
• lecho volumen: 1 mL
• columna D.I.: 7 mm
• Matriz: 6% cross-linked agarose
• tamaño de partícula: 45-165 μm
• diámetro promedio: 90 μm
• cleaning: 2-14(Ni²⁺-stripped medium.)
• Intervalo de trabajo: 3-12(Ni²⁺-stripped medium.)
• capacidad: ~40 mg binding capacity(histidine-tagged protein)
• idoneidad: suitable for bioprocess medium

Descripción

Descripción general

HisTrap^™ FF is a ready-to-use column, prepacked with precharged Ni Sepharose^™ 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).

Ni Sepharose^™ 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni²⁺ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni²⁺ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

The high flow rates made possible by the Ni Sepharose^™ 6 Fast Flow matrix make HisTrap^™ FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap^® IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.

Características y beneficios

• High binding capacity, approx. 40 mg/mL medium.
• Negligible leakage of Ni²⁺.
• Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Otras notas

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Información legal

HiTrap is a registered trademark of Cytiva

HisTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Información de seguridad

• Pictogramas: GHS09
• Palabra de señalización: Warning
• Frases de peligro: H226
• Consejos de prudencia: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Código de clase de almacenamiento: 3 - Flammable liquids