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Key Documents

GE17-5195-01

HiTrap® Phenyl High Performance

Cytiva 17-5195-01, pack of 5 × 5 mL

Sinónimos:

HiTrap Phenyl Column, Phenyl High Performance

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

ligand

phenyl

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 17-5195-01

parameter

42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

6% cross-linked agarose

particle size

24-44 μm

avg. part. size

34 μm

cleaning

2-14

working range

3-13

suitability

suitable for bioprocess medium (BioProcess medium.)
suitable for bioprocess medium

General description

HiTrap® Phenyl HP are prepacked Phenyl Sepharose™ High Performance columns for high-resolution hydrophobic interaction chromatography (HIC). The columns are used for small-scale protein purification as well as for screening of binding and elutions conditions.

Features and Benefits

  • Provide convenient and fast small-scale protein purifications.
  • Phenyl Sepharose™ High Performance is a well-established aromatic HIC medium, designed for intermediate and polishing steps where high resolution has priority.
  • Flexible design for use with syringes, single laboratory pump, or chromatography system such as AKTA design.
  • High flow rates and high capacities.
  • Excellent and easy scale-up achieved by connecting several HiTrap® columns in series or using HiPrep™ columns.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiPrep is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

related product

Referencia del producto
Descripción
Precios

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Salt Selection & Buffer Preparation

Optimize salt conditions for protein binding and impurity passage in chromatography to achieve required selectivity.

Salt Selection & Buffer Preparation

Optimize salt conditions for protein binding and impurity passage in chromatography to achieve required selectivity.

Salt Selection & Buffer Preparation

Optimize salt conditions for protein binding and impurity passage in chromatography to achieve required selectivity.

Salt Selection and Buffer Preparation

Optimize salt conditions for protein binding and impurity passage in chromatography to achieve required selectivity.

Ver todo

Protocolos

Sepharose® High Performance: Purification with High Resolution

Here we discuss purification options when working with Sepharose® High Performance and we present some examples of media screening, capture and purification.

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from Cytiva.

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico