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Merck

GE17-0756-05

Glutathione Sepharose 4B

Cytiva 17-0756-05, pack of 100 mL

Sinónimos:

affinity chromatography resin, GST-tagged proteins purification

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

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ligand

glutathione

packaging

pack of 100 mL

manufacturer/tradename

Cytiva 17-0756-05

matrix

4% agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

4-13

working range

4-13

capacity

>25 mg binding capacity (horse liver GST/ml medium)

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Este artículo
S9761T0273A6220
solubility

chloroform: 50 mg/mL, clear, colorless

solubility

methanol: 50 mg/mL, clear, colorless

solubility

acetic acid: 10 mg/mL, clear, colorless

solubility

DMSO/DMF: 1 mg/mL

form

powder

form

powder

form

powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Glutathione Sepharose 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.

Features and Benefits

  • High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
  • Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

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Warning

hcodes

Storage Class

3 - Flammable liquids


Certificados de análisis (COA)

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Artículos

This page addresses common problems associated with the expression and growth of GST-tagged proteins using Cytiva products.

This page shows how to purify GST-tagged proteins using GST SpinTrap™ from Cytiva.

This page describes general considerations for purification of GST-tagged proteins using Cytiva products.

GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

Ver todo

Protocolos

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.

Ver todo

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