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CS0780

Sigma-Aldrich

β-N-Acetylglucosaminidase Assay Kit

sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)

Sinónimos:

Beta-NAG Activity Assay Kit

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About This Item

EC Number:
222-398-7
UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 50 reactions (1 mL)
 sufficient for 500 reactions (100 μL)

Quality Level

200

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... NAGLU(4669)

Application

The kit provides the reagents required for a fast and convenient detection of β-N-Acetylglucosaminidase activity in cell lysates, tissue homogenates or purified enzyme. It is a useful tool for the detection of lysosomes in fractionated cell/tissue samples.

Biochem/physiol Actions

N-acetyl-D-glucosaminidase (NAG) in mammals is a lysosomal enzyme, which takes part in the intracellular degradation of glycolipids and glycoproteins. High activities of this enzyme have been detected in human kidney, lung and liver lysosomes. Elevated levels of serum NAG are associated with certain forms of cancer.

Analysis Note

The kit assay is based on the hydrolysis of NAG substrate (NP-GLcNAc) by the enzyme. The enzymatic hydrolysis of the substrate liberates p-nitrophenylate ion. The reaction product is detected colorimetrically at 405 nm.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Solo componentes del kit

Referencia del producto
Descripción
  • Dilution Buffer 8 mL
  • 4-Nitrophenyl-N-acetyl-β-D-glucosaminide 50 mg
  • Citrate Buffer Solution, 0.09 M 100 mL
  • p-Nitrophenol Standard Solution, 10 mM 1 mL
  • β-N-Acetylglucosaminidase from Jack beans 1 vial
  • Sodium carbonate 5 g

Related product

Referencia del producto
Descripción
Precios

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

Hazard Classifications

Carc. 2 - Eye Irrit. 2 - STOT RE 2 Oral

target_organs

Liver,Kidney

Storage Class

11 - Combustible Solids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Shanshan Wang et al.
Biochemical and biophysical research communications, 483(1), 45-51 (2017-01-09)
Activation of endothelial cells plays a key role in septic acute kidney injury (AKI). This study investigated the role of miRNA in endothelial-induced tubular cell injury in sepsis. Circulating endothelial cells (CECs) from septic AKI, non-septic AKI, septic non-AKI patients
Miguel A Lanaspa et al.
Journal of the American Society of Nephrology : JASN, 25(11), 2526-2538 (2014-05-31)
Diabetes is associated with activation of the polyol pathway, in which glucose is converted to sorbitol by aldose reductase. Previous studies focused on the role of sorbitol in mediating diabetic complications. However, in the proximal tubule, sorbitol can be converted
Qiuyuan Yin et al.
The Journal of cell biology, 219(8) (2020-07-15)
Lysosomes are degradation and signaling organelles that adapt their biogenesis to meet many different cellular demands; however, it is unknown how lysosomes change their numbers for cell division. Here, we report that the cyclin-dependent kinases CDK4/6 regulate lysosome biogenesis during
Di Chen et al.
Developmental cell, 55(5), 588-602 (2020-12-09)
Liquid-liquid phase separation (LLPS) compartmentalizes transcriptional condensates for gene expression, but little is known about how this process is controlled. Here, we showed that depletion of IPMK, encoding inositol polyphosphate multikinase, promotes autophagy and lysosomal function and biogenesis in a
Xiaoting Chen et al.
Journal of cellular and molecular medicine, 23(6), 4290-4300 (2019-04-12)
Impaired autophagic degradation of intracellular lipids is causally linked to the development of non-alcoholic steatohepatitis (NASH). Pharmacological agents that can restore hepatic autophagic flux could therefore have therapeutic potentials for this increasingly prevalent disease. Herein, we investigated the effects of
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