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Merck

C8210

Sigma-Aldrich

CHES

BioXtra, ≥99.0% (titration)

Sinónimos:

Ácido 2-(ciclohexilamino)etanosulfónico

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About This Item

Fórmula empírica (notación de Hill):
C8H17NO3S
Número de CAS:
Peso molecular:
207.29
Beilstein/REAXYS Number:
2967601
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25

product line

BioXtra

Quality Level

assay

≥99.0% (titration)

form

powder

impurities

≤0.0005% Phosphorus (P)
≤0.1% Insoluble matter

ign. residue

≤0.1%

pH

3.0-5.0 (0.5 M in H2O)

useful pH range

8.6-10.0

pKa (25 °C)

9.3

solubility

H2O: 0.5 M, clear, colorless

anion traces

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.0005%
Ca: ≤0.001%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.01%
Pb: ≤0.001%
Zn: ≤0.0005%

absorption

≤0.05 at 280 in H2O at 0.5 M
≤0.08 at 260 in H2O at 0.5 M

application(s)

diagnostic assay manufacturing

SMILES string

OS(=O)(=O)CCNC1CCCCC1

InChI

1S/C8H17NO3S/c10-13(11,12)7-6-9-8-4-2-1-3-5-8/h8-9H,1-7H2,(H,10,11,12)

InChI key

MKWKNSIESPFAQN-UHFFFAOYSA-N

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General description

CHES (2-(Cyclohexylamino) ethane sulfonic acid) is a derivative of amino acid taurine. It serves as a standard physiological buffer in the pH range of 8.5-9.5.

Application

CHES may be used:
  • for pH adjustments and as a assay buffer in catechol oxidase activity measurements(32)
  • as a buffer in electron paramagnetic resonance (EPR) spectroscopy and kinetic measurements(33)
  • as a buffer for soil solution equivalent (SSE) yeast dextrose medium for screening pH optima for growth of Aridibacter nitratireducens A24_SHP-5_238T(34)

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


Certificados de análisis (COA)

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Buffers for the physiological pH range: Acidic dissociation constants of zwitterionic compounds (ACES and CHES) in water from 5 to 55? C
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Journal of Chemical and Engineering Data, 42(1), 41-44 (1997)
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Electrophoresis, 24(17), 2887-2895 (2003-09-16)
A systematic evaluation of continuous flow-counterbalanced capillary electrophoresis for microscale preparative applications is presented. The success of the technique was found to depend on the specific nature of the analyte and background electrolyte and was most heavily influenced by factors
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The suitability of relatively slow (low absolute value of mobility) coanionic buffers in background electrolytes (BGEs) for indirect photometric detection of anions by capillary electrophoresis was investigated. As a model system, 2-(cyclohexylamino)ethanesulfonic acid (CHES) was used to buffer the indirect
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Several glycosphingolipids were labeled with the fluorphore Bodipy-Fl and analyzed using capillary electrophoresis with laser-induced fluorescence detection. GM1-, LacCer-, and Cer-Bodipy-Fl were prepared through acylation using the N-hydroxysuccinimide ester of Bodipy-Fl. Several other glycosphingolipids including GT1a-, GD1a-, GM2-, GM3-, GD3-
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In order to develop a high-throughput assay for nitric oxide metabolites, nitrite (NO2-) and nitrate (NO3-), in biological fluids, we have investigated the simultaneous determination of them using an electrophoretic lab-on-a-chip (microchip capillary electrophoresis, MCE) technique. In this study, in

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