Saltar al contenido
Merck
Todas las fotos(1)

Documentos clave

C2272

Sigma-Aldrich

Concanavalin A from Canavalia ensiformis (Jack bean)

Concanavalin A from Canavalia ensiformis (Jack bean)

biotin conjugate, Type IV, lyophilized powder

Sinónimos:

ConA

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad

biological source

Canavalia ensiformis

Quality Level

conjugate

biotin conjugate

type

Type IV

form

lyophilized powder

composition

Protein, ~85% ε1%/280

extent of labeling

4-8 mol biotin per mol protein

impurities

Carbohydrate, essentially free

storage temp.

2-8°C

¿Está buscando productos similares? Visita Guía de comparación de productos

General description

Concanavalin A is isolated from the from Canavalia ensiformis (Jack bean). It is a mitogenic lectin with a dome-like structures and is a tetrameric protein.[1]

Application

Concanavalin A from Canavalia ensiformis (Jack bean) has been used:
  • in the colabelling of fischer rat thyroid (FRT) epithelia(104)
  • to induce autoimmune hepatitis in Cre-conditional atg7 knockout mice(105)
  • to detect glycoproteins containing α-D-mannose, α-D-glucose when used with avidin or streptavidin detection system(106)

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
Concanavalin A (Con A) bound to glycoenzyme has wide applications and can be dissociated in the presence of high sugar levels.[2] Con A induces hepatic inflammation.[3] It stimulates macrophages and promotes cytokine production. Con A also exhibits anti-hepatoma effect and could be used effectively as a vaccine for treating cancer.[4]

Packaging

Package size based on protein content

Physical form

Contains sodium citrate

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

pictograms

Exclamation mark

signalword

Warning

hcodes

pcodes

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Structure and function of concanavalin A
Reeke GN, et al.
Advances in Experimental Medicine and Biology, 55, 13-33 (1975)
Atg7 knockdown augments concanavalin A-induced acute hepatitis through an ROS-mediated p38/MAPK pathway
Zhuang Y, et al.
PLoS ONE, 11(3), e0149754-e0149754 (2016)
Concanavalin A: a useful ligand for glycoenzyme immobilization?a review
Saleemuddin, M and Husain Q
Enzyme and Microbial Technology, 13(4), 290-295 (1991)
Yasuhiro Fujiwara et al.
PloS one, 16(11), e0259846-e0259846 (2021-11-17)
Epigenome research has employed various methods to identify the genomic location of proteins of interest, such as transcription factors and histone modifications. A recently established method called CUT&Tag uses a Protein-A Tn5 transposase fusion protein, which cuts the genome and
Hillary Claire Tunggal et al.
PloS one, 16(6), e0253265-e0253265 (2021-06-18)
A therapeutic vaccine that induces lasting control of HIV infection could eliminate the need for lifelong adherence to antiretroviral therapy. This study investigated a therapeutic DNA vaccine delivered with a single adjuvant or a novel combination of adjuvants to augment

Questions

  1. What agent should be used to resolve conA powder? Is this type of conA suitable for isolating the proteins on the surface of the plasma membrane? What concentration of conA should be used in IP experiments for isolating plasma membrane surface proteins?

    1 answer
    1. This product is soluble in water at a concentration of 2 mg/ml, resulting in a clear solution. Concanavalin A from Canavalia ensiformis (Jack bean) has been utilized in the colabeling of Fischer rat thyroid epithelial cells, to induce autoimmune hepatitis in Cre-conditional atg7 knockout mice, and to detect glycoproteins containing α-D-mannose and β-D-glucose when used with an avidin or streptavidin detection system. This product has not been tested or validated for immunoprecipitation experiments. It is recommended to consult literature for procedures to isolate plasma membrane surface proteins using Concanavalin A.

      Helpful?

Reviews

Active Filters

  1. Miami FL
    • Review 1
    • Votes 0
    5 out of 5 stars.

    Performed grear

    Product works well with Thermo DynaBeads for CUT&Tag/CUT&RUN assays for binding nuclei.

    Helpful?

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico