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Merck

B9673

Sigma-Aldrich

1-Bromo-3-chloropropane

for isolation of RNA

Sinónimos:

1-Bromo-3-chloropropane, I-BCP, Trimethylene bromochloride, Trimethylene chlorobromide

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About This Item

Fórmula lineal:
Cl(CH2)3Br
Número de CAS:
Peso molecular:
157.44
Beilstein/REAXYS Number:
605278
EC Number:
MDL number:
UNSPSC Code:
41105515
PubChem Substance ID:
NACRES:
NA.52

grade

for molecular biology

Quality Level

assay

≥98.5% (GC)

form

liquid

refractive index

n20/D 1.486 (lit.)

bp

144-145 °C (lit.)

density

1.592 g/mL at 25 °C (lit.)

storage temp.

room temp

SMILES string

ClCCCBr

InChI

1S/C3H6BrCl/c4-2-1-3-5/h1-3H2

InChI key

MFESCIUQSIBMSM-UHFFFAOYSA-N

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General description

BCP can be used in place of chloroform and is less toxic. The use of BCP in the single-step TRI reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA. 1-Bromo-3-chloropropane is a phase separation reagent used in protocols for isolation of nucleic acids.

Application

1-Bromo-3-chloropropane has been used in molecular biology applications for the isolation of high quality RNA from:
  • feline teeth
  • mice brain and liver tissues
  • human ectocervical epithelial cells
  • placental samples
  • human mesenchymal stromal cells (hMSCs)

Features and Benefits

  • less toxic alternative to chloroform
  • compatible with TRI reagent for isolation of RNA with decreased DNA contamination

Other Notes

BCP can be used in place of chloroform, and is less toxic. The use of BCP in the single-step TRI Reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA.

related product

Referencia del producto
Descripción
Precios

pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Muta. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 2

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


Certificados de análisis (COA)

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Protocolos

TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.

TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.

TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.

TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.

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