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B0174

Sigma-Aldrich

AccuTaq™ LA 10× Buffer

10X Buffer for long and accurate PCR

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About This Item

UNSPSC Code:
41106306

grade

for molecular biology

Quality Level

200

form

liquid

packaging

vial of 0.5 mL

concentration

10 ×

technique(s)

PCR: suitable

storage temp.

−20°C

General description

AccuTaq™ LA 10X buffer is optimized for enhanced amplification of complex templates, when using AccuTaq long and accurate (LA) DNA polymerase.

Application

AccuTaq™ LA 10× Buffer has been used:

  • in PCR reaction mixture for the amplification of human BRCA1 gene.
  • as a component of the PCR mix for the amplification of the genomic DNA from Triticum aestivum
  • as a component of the PCR mix for the reverse transcription of RNA from foot and mouth disease virus (FMDV)

Legal Information

AccuTaq is a trademark of Sigma-Aldrich Co. LLC

Related product

Referencia del producto
Descripción
Precios

hcodes

H412

Hazard Classifications

Aquatic Chronic 3

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000295519
SLCP4419
SLCF8408
SLCB2592
SLBW4090

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Hameer Ruparel et al.
Genome research, 14(2), 296-300 (2004-02-06)
Deletion or insertion mutations lead to a frameshift that causes misalignment between wild-type and mutated allele sequences, making it difficult to identify such mutations unambiguously by using electrophoresis-based DNA sequencing. We have previously established the feasibility of an accurate DNA
Eva-Maria Ladenburger et al.
Molecular and cellular biology, 29(13), 3605-3622 (2009-04-22)
A database search of the Paramecium genome reveals 34 genes related to Ca(2+)-release channels of the inositol-1,4,5-trisphosphate (IP(3)) or ryanodine receptor type (IP(3)R, RyR). Phylogenetic analyses show that these Ca(2+) release channels (CRCs) can be subdivided into six groups (Paramecium
Baozhong Meng et al.
The Journal of general virology, 86(Pt 5), 1555-1560 (2005-04-16)
Grapevine rupestris stem pitting-associated virus (GRSPaV), a member of the genus Foveavirus within the family Flexiviridae, is the putative causal agent of the disease Rupestris stem pitting (RSP) of grapevines. GRSPaV comprises a family of variants whose pathological characteristics have
David T Hughes et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(21), 9831-9836 (2010-05-12)
The mammalian gastrointestinal (GI) tract is colonized by a complex consortium of bacterial species. Bacteria engage in chemical signaling to coordinate population-wide behavior. However, it is unclear if chemical sensing plays a role in establishing mammalian host-bacterial commensal relationships. Enterohemorrhagic
Jeong-Yun Choi et al.
The Journal of biological chemistry, 286(36), 31180-31193 (2011-07-26)
The hyperthermophilic crenarchaeon Sulfolobus solfataricus P2 encodes three B-family DNA polymerase genes, B1 (Dpo1), B2 (Dpo2), and B3 (Dpo3), and one Y-family DNA polymerase gene, Dpo4, which are related to eukaryotic counterparts. Both mRNAs and proteins of all four DNA
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Protocolos

Long & Accurate PCR with RedAccuTaq®

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

Long & Accurate PCR with RedAccuTaq®

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

Long & Accurate PCR with RedAccuTaq®

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

Long & Accurate PCR with RedAccuTaq®

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

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