Saltar al contenido
Merck
Todas las fotos(2)

Documentos clave

Ver Toda la documentación

A3547

Sigma-Aldrich

Achromopeptidase from bacteria

lyophilized powder, ≥1,000 units/mg solid

Sinónimos:

ACP, Lysyl endopeptidase

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(2)

About This Item

Código UNSPSC:
12352204
NACRES:
NA.54

origen biológico

bacterial (LYSOBACTER SP. BACTERIA M497-1)

Nivel de calidad

200

Formulario

lyophilized powder

actividad específica

≥1,000 units/mg solid

mol peso

27 kDa

técnicas

cell based assay: suitable

idoneidad

suitable for cell lysis

aplicaciones

diagnostic assay manufacturing

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Aplicación

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections.

Acciones bioquímicas o fisiológicas

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD600 = 0.6 over 2 hours at 37 °C.
Achromopeptidase is a lysyl endopeptidase with a MW of ~27 kDa. It is useful for lysis of Gram-positive bacteria that are resistant to lysozyme.
pH Optimum for activity: pH 8.5 - 9
Approximately 500-1,500 un/ml achromopetidase can be used to lyse cells at a density of OD600=0.6 over 2 hours at 37 °C.

Definición de unidad

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

Forma física

crude powder

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H334

Consejos de prudencia

P261 - P342 + P311

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
0000429881
0000429881
0000412968
0000412968
0000394744

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

Busque por un COA

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Disposable platform for bacterial lysis and nucleic acid amplification based on a single USB-powered printed circuit board.
Shah, et al.
PLoS ONE, 18, e0284424-e0284424 (2023)
Georgios Chondrogiannis et al.
Scientific reports, 11(1), 6140-6140 (2021-03-19)
Enzymes are the cornerstone of modern biotechnology. Achromopeptidase (ACP) is a well-known enzyme that hydrolyzes a number of proteins, notably proteins on the surface of Gram-positive bacteria. It is therefore used for sample preparation in nucleic acid tests. However, ACP
Adebayo Shittu et al.
Journal of medical microbiology, 53(Pt 1), 51-55 (2003-12-10)
The isolation, molecular identification and genotyping of multiresistant Staphylococcus sciuri and Staphylococcus haemolyticus from skin and soft-tissue infections are reported. Accurate and full identification of three coagulase-negative staphylococcal isolates was achieved using PCR, while the API STAPH method failed to
Chelsey A Smith et al.
Lab on a chip, 23(3), 451-465 (2022-12-24)
Cervical cancer is a leading cause of cancer death for women in low-resource settings. The World Health Organization recommends that cervical cancer screening programs incorporate HPV DNA testing, but available tests are expensive, require laboratory infrastructure, and cannot be performed
J R Buser et al.
Analytical methods : advancing methods and applications, 8(14), 2880-2886 (2016-04-14)
Sample preparation, including bacterial lysis, remains a hurdle in the realization of complete point-of-care tests for many pathogens. Here, we developed a sample preparation methodology for enzymatic lysis and sample heating for low-resource, point-of-care applications. We show an instrument-free chemical
Ver todos los artículos relacionados

Protocolos

Enzymatic Assay of Achromopeptidase

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

Enzymatic Assay of Achromopeptidase

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

Enzymatic Assay of Achromopeptidase

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

Contenido relacionado

Reactivos e intensificadores de lisis de células completas

Kits de extracción de proteínas, tampones de lisis celular y reactivos para la solubilización de proteínas de cultivos bacterianos, de levaduras e insectos, así como de cultivos celulares de plantas y mamíferos y muestras de tejidos.

Cell Lysis Buffers & Protein Extraction Reagents

Find cell lysis and protein extraction reagents like cell lysis buffers, protein extraction kits, extraction enzymes, and other lysis enhancers for isolating and purifying proteins.

Reactivos e intensificadores de lisis de células completas

Kits de extracción de proteínas, tampones de lisis celular y reactivos para la solubilización de proteínas de cultivos bacterianos, de levaduras e insectos, así como de cultivos celulares de plantas y mamíferos y muestras de tejidos.

Reactivos e intensificadores de lisis de células completas

Kits de extracción de proteínas, tampones de lisis celular y reactivos para la solubilización de proteínas de cultivos bacterianos, de levaduras e insectos, así como de cultivos celulares de plantas y mamíferos y muestras de tejidos.

Ver todo

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico