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23184

Sigma-Aldrich

Fmoc chloride

BioReagent, ≥99.0% (HPLC)

Sinónimos:

9-Fluorenylmethoxycarbonyl chloride, 9-Fluorenylmethyl chloroformate, Fmoc-Cl

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About This Item

Fórmula empírica (notación de Hill):
C15H11ClO2
Número de CAS:
28920-43-6
Peso molecular:
258.70
Beilstein/REAXYS Number:
2279177
EC Number:
249-313-6
MDL number:
MFCD00001138
UNSPSC Code:
12352108
PubChem Substance ID:
329752391
NACRES:
NA.22

product line

BioReagent

Quality Level

100

assay

≥99.0% (HPLC)

form

solid

mp

61-64 °C
62-64 °C (lit.)

functional group

Fmoc

storage temp.

2-8°C

SMILES string

ClC(=O)OCC1c2ccccc2-c3ccccc13

InChI

1S/C15H11ClO2/c16-15(17)18-9-14-12-7-3-1-5-10(12)11-6-2-4-8-13(11)14/h1-8,14H,9H2

InChI key

IRXSLJNXXZKURP-UHFFFAOYSA-N

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Application

9-Fluorenylmethoxycarbonyl chloride (Fmoc-Cl) is a reagent for pre-column derivatization of amino acids and biogenic amines for HPLC amino acid analysis and for preparing N-Fmoc amino acids for solid-phase peptide synthesis.
Reagent for derivatizing amino acids for HPLC amino acid analysis and for preparing N-Fmoc amino acids for solid-phase peptide synthesis.

Other Notes

Reagent for the pre-column fluorescent derivatization of amino acids

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H314

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

supp_hazards

EUH029

Storage Class

8A - Combustible corrosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Sigma-Aldrich

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Ftaldialdehído suitable for HPLC fluorimetric detection of amino acids, ≥99% (HPLC), powder or crystals

Sigma-Aldrich

P0657

Ftaldialdehído

E J Miller et al.
Analytical biochemistry, 190(1), 92-97 (1990-10-01)
A recently described procedure for amino acid analyses has been modified and adapted for use in quantitating the unique mixture of products commonly found in hydrolysates of the collagens. The method involves precolumn derivatization of hydrolysates with 9-fluorenylmethyl chloroformate (FMOC-CL)
Ibolya Molnár-Perl
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(17-18), 1241-1269 (2011-03-05)
An overview is presented on the advancement of the two most frequently used derivatization protocols applying the o-phthalaldehyde (OPA)-thiol and the fluorenylmethyloxycarbonyl (FMOC) chloride reagents, prior to the high performance liquid chromatographic analysis of amino acids. This review pays special
Valentin Lozanov et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 860(1), 92-97 (2007-11-07)
A liquid chromatography method for simultaneous analysis of amino acids, polyamines, catecholeamines and metanephrines in human body fluids after derivatization with 9-fluorenylmethyloxycarbonyl chloride was developed. The chromatographic behavior of analytes at different pH of mobile phase was studied. Successful baseline
P Fürst et al.
Journal of chromatography, 499, 557-569 (1990-01-19)
Reversed-phase high-performance liquid chromatography (RP-HPLC) is a powerful method for assaying physiological amino acid concentrations in biological fluids. Four pre-column derivatization methods, with o-phthaldialdehyde (OPA), 9-fluorenylmethyl chloroformate (FMOC-Cl), phenyl isothiocyanate (PITC) and 1-dimethylaminonaphthalene-5-sulphonyl chloride (dansyl-Cl), were assessed with respect to
A Jámbor et al.
Journal of chromatography. A, 1216(34), 6218-6223 (2009-07-28)
This paper, as a novelty to this field, presents the deproteinization and derivatization of plasma's free amino acids (PFAAs), simultaneously, in a single step, with the acetonitrile (ACN) containing 9-fluorenylmethyloxycarbonyl chloride (FMOC) reagent. Deproteinization and derivatization, were studied with 22
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