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Merck

230251

Sigma-Aldrich

Eosin Y

75% (HPLC)

Sinónimos:

2′,4′,5′,7′-Tetrabromofluorescein, Acid Red 87, Bromo acid J. TS, XL, or XX, Bromofluorescein, Bronze Bromo ES, Eosin yellowish, Solvent red 43

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About This Item

Fórmula empírica (notación de Hill):
C20H8Br4O5
Número de CAS:
Peso molecular:
647.89
Colour Index Number:
45380:2
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

Quality Level

assay

75% (HPLC)

form

powder

color

orange to very dark orange

solubility

spirit: soluble

λmax

524 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1c(Br)cc2c(Oc3c(Br)c(O)c(Br)cc3C24OC(=O)c5ccccc45)c1Br

InChI

1S/C20H8Br4O5/c21-11-5-9-17(13(23)15(11)25)28-18-10(6-12(22)16(26)14(18)24)20(9)8-4-2-1-3-7(8)19(27)29-20/h1-6,25-26H

InChI key

DBZJJPROPLPMSN-UHFFFAOYSA-N

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General description

Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.

Application

Eosin Y has been used as a reagent in the preparation of Nigrosin-Eosin (NE) stain and is used in hematoxylin and eosin (H&E) staining technique to visualize alginate.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Transplantation, 94(11), 1172-1177 (2012)
Electrophoretic detection of alginate by Hematoxylin and Eosin fluorescence: Implications in cell encapsulation/tissue engineering
Verma Y K
Reactive functional Polymers, 102, 130-136 (2016)
John D. Bancroft
Theory and Practice of Histological Techniques (2008)

Protocolos

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