199664

Sudan Black B

certified by the Biological Stain Commission

• Sinónimos: Ceres black BN, Fat Black HB, Solvent Black 3
• Fórmula empírica (notación de Hill): C₂₉H₂₄N₆
• Número de CAS: 4197-25-5
• Peso molecular: 456.54
• Número de índice de color: 26150
• Beilstein: 723248
• Número CE: 224-087-1
• Número MDL: MFCD00006919
• Código UNSPSC: 12171500
• ID de la sustancia en PubChem: 24851932
• NACRES: NA.47

Propiedades

• grado: certified by the Biological Stain Commission
• Nivel de calidad: 200
• Formulario: powder
• técnicas: microbe id | staining: suitable
• color: dark brown to very dark brown
• mp: 120-124 °C (lit.)
• λmáx.: 415 nm (2nd); 598 nm
• ε (coeficiente de extinción): ≥20000 at 596-603 nm in ethanol
• aplicaciones: diagnostic assay manufacturing; hematology; histology
• temp. de almacenamiento: room temp
• cadena SMILES: CC1(C)Nc2cccc3c(ccc(N1)c23)\N=N\c4ccc(\N=N\c5ccccc5)c6ccccc46
• InChI: 1S/C29H24N6/c1-29(2)30-26-14-8-13-22-25(17-18-27(31-29)28(22)26)35-34-24-16-15-23(20-11-6-7-12-21(20)24)33-32-19-9-4-3-5-10-19/h3-18,30-31H,1-2H3/b33-32+,35-34+
• Clave InChI: YCUVUDODLRLVIC-VPHDGDOJSA-N

Descripción

Aplicación

Sudan Black B has been used in brain BLAQ (Block Lipids and Aldehyde Quench) technique and is used in lipid detection.

Acciones bioquímicas o fisiológicas

Sudan Black B has been used to stain phospholipids and intracellular lipids. Staining pattern is almost similar to myeloperoxidase reaction, with positive staining of granulocytic cells, eosinophils and weak monocytic staining.It doesn′t stain lymphocytes. Sudan Black B can be used to stain older blood or bone marrow smears, but the stain slightly fades over time. It is considered to be a most sensitive and specific reagent for lipid staining. Sudan Black B stains phospholipids gray and neutral fats blue-black.

Idoneidad

Certified as a fat staining using the Churukian variant of the Lillie-Ashburn supersaturated isopropanol method. Sudan III is substituted for the oil red O in the procedure. Burdon′s stain for bacterial lipid.

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Warning
• Frases de peligro: H341
• Consejos de prudencia: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Clasificaciones de peligro: Muta. 2
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)