11965085001
Roche
Anti-His6-Peroxidase
from mouse IgG1
Sinónimos:
antibody
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About This Item
Código UNSPSC:
12352203
Productos recomendados
origen biológico
mouse
Nivel de calidad
conjugado
peroxidase conjugate
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
BMG-his-1, monoclonal
Formulario
lyophilized
envase
pkg of 50 U
fabricante / nombre comercial
Roche
isotipo
IgG1
temp. de almacenamiento
2-8°C
Descripción general
Anti-His6-Peroxidase is a monoclonal antibody to His6-tagged proteins, conjugated to horseradish peroxidase. Anti-His6-Peroxidase specifically recognizes an epitope of six consecutive histidine residues of both natural and recombinant proteins. The antibody reacts with native and denatured histidine-tagged fusion proteins independent of the epitope-sequence location; however, it preferentially recognizes the C-terminal His6 epitope with high sensitivity.
Especificidad
Anti-His6-Peroxidase specifically recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of location of the epitopesequence. However, Anti-His6-Peroxidase preferentially recognizes the C-terminal His6 epitope with high sensitivity. Anti-His6 allows specific and sensitive detection of histidine-tagged proteins irrespective of the expression system used.
The antibody recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of the epitope-sequence location.
Aplicación
Anti-His6-Peroxidase has been used in in vitro serum stability of fusion toxins, pull-down assay and western blotting.
Anti-His6-Peroxidase is used for the detection of His6-tagged proteins in:
- ELISA (enzyme-linked immunosorbent assay)
- Western blot
Calidad
Function test: Western blot using extracts from cell line expressing a recombinant His6-tagged protein.
Nota de preparación
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
The following concentrations should be taken as a guideline:
- ELISA: 100 mU/ml
- Western blot: 100mU/ml
Reconstitución
Add 1 ml double-distilled water to a final concentration of 50 U/ml.
Reconstitution should be performed for at least 10 minutes.
Reconstitution should be performed for at least 10 minutes.
Otras notas
For life science research only. Not for use in diagnostic procedures.
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Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Skin Sens. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
does not flash
Punto de inflamabilidad (°C)
does not flash
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