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Merck

10107921001

Roche

Nuclease S7

Micrococcal nuclease, from Staphylococcus aureus

Sinónimos:

s7 nuclease

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About This Item

UNSPSC Code:
12352204

biological source

Staphylococcus aureus

Quality Level

form

lyophilized

specific activity

~15,000 U/mg

packaging

pkg of 15,000 U

manufacturer/tradename

Roche

optimum pH

7.5

shipped in

wet ice

Application

  • Removal of endogenous RNA from in vitro translation systems
  • RNA sequencing experiments
Nuclease S7 has been used in ribonuclease selection process for ribosome profiling.

Biochem/physiol Actions

Nuclease S7 is an endonuclease that mainly cleaves single-stranded substrates. It also cleaves double-stranded DNA or RNA. It cleaves the 5′-phosphodiester bonds of RNA and DNA to yield 3′-mononucleotides and oligonucleotides. Activity of the enzyme is dependent on Ca2+ and can be completely inactivated by the addition of EDTA. Nuclease S7 cleavage is highly specific to AT rich region than GC on the DNA.

Unit Definition

One unit releases a sufficient amount of acid-soluble oligonucleotides to produce an increase of 1.0 at A260.

Storage and Stability

Store unopened reagent at 2-8 °C; when diluted to 1 mg/mL, store at -15 to -25 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

pictograms

Exclamation markHealth hazard

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

High sequence specificity of micrococcal nuclease.
Dingwall C, et al.
Nucleic Acids Research, 9(12), 2659-2674 (1981)
Organ-specific translation elongation rates measured by in vivo ribosome profiling.
Gerashchenko M V, et al.
bioRxiv, 279257 (2018)
Sequence specific cleavage of African green monkey ?-satellite DNA by micrococcal nuclease.
Horz W, et al.
Nucleic Acids Research, 11(13), 4275-4285 (1983)
Maxim V Gerashchenko et al.
Nucleic acids research, 49(2), e9-e9 (2020-12-03)
There has been a surge of interest towards targeting protein synthesis to treat diseases and extend lifespan. Despite the progress, few options are available to assess translation in live animals, as their complexity limits the repertoire of experimental tools to
Ribonuclease selection for ribosome profiling.
Gerashchenko M V and Gladyshev V N
Nucleic Acids Research, 45(2), e6-e6 (2016)

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