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MAB1254B

Sigma-Aldrich

Anti-Chymase Antibody, clone B7, biotin conjugated

clone B7, Chemicon®, from mouse

Sinónimos:

Mast Cell Protease I

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

conjugado

biotin conjugate

forma del anticuerpo

culture supernatant

tipo de anticuerpo

primary antibodies

clon

B7, monoclonal

reactividad de especies

human

fabricante / nombre comercial

Chemicon®

técnicas

dot blot: suitable
immunohistochemistry: suitable

isotipo

IgG1κ

idoneidad

not suitable for Western blot

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... CMA1(1215)

Descripción general

Mast cell chymase (EC 3.4.21.39), a member of the granzyme subfamily of the S1 family of peptidases, cleaves C-terminal to Phenylalanine, Tyrosine, Tryptophan, and Leucine residues (in order of preference). Chymase is stored in Mast cell granules within the cell and is the major secreated Mast cell protease. Secreated Mast cell chymase is believed to be involved in extracellular matrix degradation, vasoactive peptide generation, and regulation of gland secreation.

Especificidad

Reacts with human mast cell chymase.

Inmunógeno

Chymase purified from human skin.

Aplicación

Immunohistochemistry at 1:2,000:1:4,000.

For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.

Not recommended for use on Western blots.

Optimal working dilutions must be determined by end user.
Research Category
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms
This Anti-Chymase Antibody, clone B7, biotin conjugated is validated for use in DB, IH for the detection of Chymase.

Forma física

Culture supernatant, purified by ammonium sulfate precipitation and DEAE-Sephacyl chromatography, labeled with biotin. In 0.01M PBS, 15 mg/mL BSA, 0.1% sodium azide, pH 7.1.

Almacenamiento y estabilidad

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

Nota de análisis

Control
POSITIVE CONTROL: lung, heart, skin, placenta.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Arnold S Kirshenbaum et al.
PloS one, 11(7), e0159177-e0159177 (2016-07-28)
Hermansky-Pudlak Syndrome type-1 (HPS-1) is an autosomal recessive disorder caused by mutations in HPS1 which result in reduced expression of the HPS-1 protein, defective lysosome-related organelle (LRO) transport and absence of platelet delta granules. Patients with HPS-1 exhibit oculocutaneous albinism
J H McKerrow et al.
Molecular medicine (Cambridge, Mass.), 6(5), 450-460 (2000-08-22)
Proteases facilitate several steps in cancer progression. To identify proteases most suitable for drug targeting, actual enzyme activity and not messenger RNA levels or immunoassay of protein is the ideal assay readout. An automated microtiter plate assay format was modified
Narcy Arizmendi et al.
Nanomaterials (Basel, Switzerland), 11(4) (2021-05-01)
Biodegradable polymeric nanoparticles (NPs) such as poly(lactic-co-glycolic acid) (PLGA) and polyvinyl alcohol (PVA) have been used as drug delivery systems for natural and synthetic compounds and are designed to control the loading and release of biodegradable materials to target cells
Jiusong Sun et al.
Circulation, 120(11), 973-982 (2009-09-02)
Mast cell chymase may participate in the pathogenesis of human abdominal aortic aneurysm (AAA), yet a direct contribution of this serine protease to AAA formation remains unknown. Human AAA lesions had high numbers of chymase-immunoreactive mast cells. Serum chymase level

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