MAB1254

Anti-Chymase Antibody, clone B7

clone B7, 2.4 mg/mL, Chemicon^®

• Sinónimos: Mast Cell Protease I
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: B7, monoclonal
• reactividad de especies: human
• fabricante / nombre comercial: Chemicon^®
• concentración: 2.4 mg/mL
• técnicas: dot blot: suitable; immunohistochemistry: suitable
• isotipo: IgG1κ
• idoneidad: not suitable for Western blot
• Nº de acceso NCBI: NM_001836.2
• Nº de acceso UniProt: P23946
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CMA1(1215)

Descripción

Descripción general

Mast cell chymase (EC 3.4.21.39), a member of the granzyme subfamily of the S1 family of peptidases, cleaves C-terminal to Phenylalanine, Tyrosine, Tryptophan, and Leucine residues (in order of preference). Chymase is stored in Mast cell granules within the cell and is the major secreated Mast cell protease. Secreated Mast cell chymase is believed to be involved in extracellular matrix degradation, vasoactive peptide generation, and regulation of gland secreation.

Especificidad

Reacts with human mast cell chymase.

Inmunógeno

Chymase purified from human skin.

Aplicación

Anti-Chymase Antibody, clone B7 is an antibody against Chymase for use in DB, IH.

Dot blotting: 0.5-1.0 μg/mL.

Immunohistochemistry: 0.5-1.0 μg/mL. For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.

Not recommended for use on Western blots.

Optimal working dilutions must be determined by end user.

Research Category
Inflammation & Immunology

Research Sub Category
Inflammation & Autoimmune Mechanisms

Forma física

Format: Purified

Purified by protein G affinity chromatography. Liquid in PBS with 20% glycerol, pH 7.4.

Almacenamiento y estabilidad

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt. Antibody may also be stored at -20ºC in undiluted aliquots.

Nota de análisis

Control
POSITIVE CONTROL: lung, heart, skin, placenta.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable