ABN101

Anti-GAD2(GAD67) Antibody

rabbit polyclonal

• Sinónimos: Glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, GAD-65, Glutamate decarboxylase 65 kDa isoform
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• Nombre del producto: Anti-GAD65 Antibody, from rabbit, purified by affinity chromatography
• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: mouse, human, rat, pig
• reactividad de especies (predicha por homología): canine (based on 100% sequence homology), porcine (based on 100% sequence homology)
• técnicas: western blot: suitable
• Nº de acceso NCBI: NP_000809
• Nº de acceso UniProt: Q05329
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... GAD2(2572)

Descripción

Descripción general

GAD65 is a group II decarboxylase enzyme that catalyzes the biosynthesis of GABA from glutamate with the aid of the cofactor, pyridoxal 5′-phosphate. GAD65 localizes at presynaptic GABAergic terminals and is reported to supply local vesicular pools of GABA facilitating transient inhibitiory neurotransmission, although GAD65 may also play a role in more sustained tonic inhibitory signals. It is present as a dimer of alpha and beta subunits and is regulated by alternative splicing, which may produce the GAD67 isoform. GAD65 also undergoes post-translational modifications such as palmitoylation of N-terminal cysteine residues and phosphorylation of multiple alpha-subunit serine residues that enable membrane-anchoring and other vesicular activities. GAD65 has been implicated in neurological conditions resulting from failure of inhibitory neurotransmission such as Huntington′s disease and epilepsy. In addition to its CNS presence, GAD65 also produces GABA in pancreatic beta cells and dysfunction in this area may contribute to the pathogenesis of insulin-dependent diabetes mellitus.

Inmunógeno

KLH-conjugated linear peptide corresponding to human GAD65.

Aplicación

Anti-GAD Antibody is an antibody against GAD65 for use in Western Blotting. See below for more information about this anti GAD antibody.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Western Blot Analysis: 1 µg/mL from a representative lot detected GAD65 on 10 µg of rat brain membrane tissue lysate. Western Blot performed without Tween.

Calidad

Evaluated by Western Blot in rat brain membrane tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected GAD65 on 10 µg of rat brain membrane tissue lysate.

Descripción de destino

~59/63 kDa observed. The calculated molecular weight is 65 kDa, however GAD65 has been shown as a ~59/63 doublet in western blots. (Christgau, S., et al. (1991). Journal of Biological Chemistry. 266(31):21257-21264.) An uncharacterized band appears at ~38 kDa in some lysates.

Forma física

Affinity purified

Purified Rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
Rat brain membrane tissue lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable