615700
Total Antioxidant Status Assay Kit
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About This Item
Código UNSPSC:
41116133
NACRES:
NA.84
Productos recomendados
uso
sufficient for 50 tests
Nivel de calidad
fabricante / nombre comercial
Calbiochem®
condiciones de almacenamiento
OK to freeze
assay range
standard curve range: 0-2.5 mM
(plasma)
entrada
sample type serum
sample type plasma
sample type tissue extract(s)
sample type beverage(s)
sample type plant extract(s)
método de detección
colorimetric
Condiciones de envío
ambient
temp. de almacenamiento
2-8°C
Descripción general
A colorimetric assay kit for the measurement of the total antioxidant status. Each kit is suitable for performing a minimum of 50 assays.
Note: Spectrophotometer equipped with a temperature-controlled cuvette holder is strongly recommended for use with this kit.
Componentes
Substrate, Chromogen, Standard, Buffer, and a user protocol.
Advertencia
Toxicity: Standard Handling (A)
Principio
The Calbiochem Total Antioxidant Status Assay Kit is designed to assay antioxidant levels in serum or plasma samples. Additionally, the assay may be used to measure the antioxidant potential of (suitably solubilized) food and drug samples.
Nota de preparación
• Chromogen: Add 10 ml Buffer to each vial of Chromogen needed for the assay (10 tests per vial). After reconstitution, the Chromogen is stable for 2 days at 4°C or 8 h at room temperature.
• Substrate: Dilute each vial of Substrate needed for the assay by adding 7.5 ml Buffer. After dilution, the substrate is stable for 24 h at 4°C. NOTE: Some automated methods do not require substrate dilution.
• Standard: Add 1 ml distilled water to each vial of Standard needed for the assay. After reconstitution, the Standard is stable for 2 days at 4°C or 1 month at -20°C.
• Substrate: Dilute each vial of Substrate needed for the assay by adding 7.5 ml Buffer. After dilution, the substrate is stable for 24 h at 4°C. NOTE: Some automated methods do not require substrate dilution.
• Standard: Add 1 ml distilled water to each vial of Standard needed for the assay. After reconstitution, the Standard is stable for 2 days at 4°C or 1 month at -20°C.
Almacenamiento y estabilidad
Upon arrival store the entire kit contents at 4°C. Note: The concentration of the standard is lot-specific. Please refer to the vial label for lot-specific concentration.
Nota de análisis
Calculation of Total Antioxidant StatusThe antioxidant concentration of the Standard is used to calculate the antioxidant levels in the samples.
1. Determine the ΔA for the samples, standard, and blank. ΔA = A - Ao
2. Calculate the concentration of antioxidants in the sample using the formula below:
<div class="Bio_doc_image">Figure 2: Antioxidant Concentration Formula
</div>
NOTE: If the antioxidant concentration is greater than 2.5 mM dilute the sample with 0.9% NaCl and re-assay. Typical antioxidant levels in normal human plasma are 1.30-1.77 mM.
1. Determine the ΔA for the samples, standard, and blank. ΔA = A - Ao
2. Calculate the concentration of antioxidants in the sample using the formula below:
<div class="Bio_doc_image">Figure 2: Antioxidant Concentration Formula
</div>
NOTE: If the antioxidant concentration is greater than 2.5 mM dilute the sample with 0.9% NaCl and re-assay. Typical antioxidant levels in normal human plasma are 1.30-1.77 mM.
Otras notas
Miller, N.J., et al. 1993. Clin. Science 84, 407.
Información legal
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Órganos de actuación
Respiratory system
Código de clase de almacenamiento
10 - Combustible liquids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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