Testing for the presence of IgG and IgM is not part of the release specs. Please refer to the CoA for the complete release specs of this product.
1.12018
Albumin fraction V
(from bovine serum) for biochemistry
Sinónimos:
Albumin fraction V, Bovine serum albumin, Serum albumin
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About This Item
Número de CAS:
MDL number:
UNSPSC Code:
12352202
EC Index Number:
292-322-5
NACRES:
NA.21
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Productos recomendados
biological source
bovine
form
solid
concentration
≥97% protein (spectralphotometric; calc. on anhydrous substance)
≥98% (Assay of Albumin (Agarose gel electrophoresis))
technique(s)
immunoassay: suitable
western blot: suitable
pH
6.8-7.2 (20 °C, 1 g/L in H2O)
solubility
1 g/L
bulk density
150 kg/m3
storage temp.
2-8°C
General description
Application
Albumin fraction V has been used:
- as a component of perfusion buffer for perfusion protocol for rodent organ perfusion [3]
- as a blocking agent in western blot analysis[4]
- as a nitrated protein standard to study the effects of antibodies against 3-nitrotyrosine and to characterize the binding and detection of nitrated proteins in one-sided and sandwich immunoassays[5]
Biochem/physiol Actions
Analysis Note
Appearance (colour): slightly yellow
Appearance (description): leafs
Assay of protein (spectralphotometric; calc. on anhydrous substance): ≥ 97.0 %
Assay of Albumin (Agarose gel electrophoresis): ≥ 98.0 %
pH-value (1 %; sodium chloride solution c = 0.5 mol/ l): 6.8 - 7.2
K (Potassium): ≤ 0.02 %
Na (Sodium): ≤ 0.5 %
Mg (Magnesium): ≤ 0.01 %
Ca (Calcium): ≤ 0.05 %
Heavy metals (as lead): ≤ 0.001 %
Fats: ≤ 0.2 %
Decomposition products: not detectable
Water (according to Karl Fischer): ≤ 5.00 %
Ash (600 °C): ≤ 1 %
NADH Oxidase (NADH; pH 7.5; 25° C): not detectable
LDH (pyruvate; pH 7.5; 25° C): not detectable
Appearance (description): leafs
Assay of protein (spectralphotometric; calc. on anhydrous substance): ≥ 97.0 %
Assay of Albumin (Agarose gel electrophoresis): ≥ 98.0 %
pH-value (1 %; sodium chloride solution c = 0.5 mol/ l): 6.8 - 7.2
K (Potassium): ≤ 0.02 %
Na (Sodium): ≤ 0.5 %
Mg (Magnesium): ≤ 0.01 %
Ca (Calcium): ≤ 0.05 %
Heavy metals (as lead): ≤ 0.001 %
Fats: ≤ 0.2 %
Decomposition products: not detectable
Water (according to Karl Fischer): ≤ 5.00 %
Ash (600 °C): ≤ 1 %
NADH Oxidase (NADH; pH 7.5; 25° C): not detectable
LDH (pyruvate; pH 7.5; 25° C): not detectable
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Artículos
BCA protein assay or Smith assay to determine protein concentration in a sample using Spectroquant® Prove photometers.
BCA protein assay or Smith assay to determine protein concentration in a sample using Spectroquant® Prove photometers.
BCA protein assay or Smith assay to determine protein concentration in a sample using Spectroquant® Prove photometers.
BCA protein assay or Smith assay to determine protein concentration in a sample using Spectroquant® Prove photometers.
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how much IgG and IgM, if any, is present in fraction V albumin? Are there trace amounts?
1 answer-
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