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09-412

Sigma-Aldrich

Anti-ISG15/UCRP Antibody

Upstate®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

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biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

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Este artículo
HPA004627SAB5701422AB1431
antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

-

antibody form

serum

species reactivity

human

species reactivity

human

species reactivity

human, mouse, rat

species reactivity

human

clone

polyclonal

clone

polyclonal

clone

monoclonal

clone

polyclonal

Gene Information

human ... ISG15(9636)

Gene Information

human ... ISG15(9636)

Gene Information

human ... ISG15(9636)

Gene Information

human ... IFNB1(3456)

technique(s)

western blot: suitable

technique(s)

immunoblotting: 0.04-0.4 μg/mL

technique(s)

immunohistochemistry: 1:50-1:200, western blot: 1:500-1:2000

technique(s)

ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, neutralization: suitable, western blot: suitable

General description

ISG15 (also called ubiquitin cross-reactive protein (UCRP) due to its cross-reactivity with ubiquitin antibodies) was originally identified as an interferon stimulated gene (ISG) whose expression is highly induced upon interferon treatment. ISG15 contains two ubiquitin homology domains in tandem and shows ~30% identity to ubiquitin. Unlike ubiquitin, that is almost 100% homologous across species, ISG15 is only conserved in dispersed regions. Homology among the five identified mammalian ISG15 proteins is only about 47%. Like other Ubls, ISG15 is synthesized as a 17-kDa precursor form and processed to 15- kDa protein by a specific protease in order to expose di-glycine residues at the carboxyl terminal which is critical for subsequent conjugation to target proteins.

Specificity

Antibody 09-412 recognizes several members of the ubiquitin-like (Ubl) family including ubiquitin, SUMO-1, SUMO-2, SUMO-3, Nedd8, FAT10, Ubl5, Urm1 and Fub1. This antibody shows no cross-reactivity with any of these Ubls in recombinant form upon Western blotting under the conditions used.

Immunogen

Peptide corresponding to residues 85-101 of human ISG15.

Application

Detect ISG15/UCRP using this Anti-ISG15/UCRP Antibody validated for use in WB.
Research Category
Protein Trafficking
Research Sub Category
Ubiquitin & Ubiquitin Metabolism

Target description

18 kDa

Physical form

100 µL of purified by salt precipitation and peptide immunochromatography in PBS containing 0.01 M sodium azide. Dilute to working strength with phosphate buffered saline pH 7.2-7.4 and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).
Format: Purified
Salt precipitation

Storage and Stability

Stable for 1 year at -20°C from date of shipment for up to 12 months.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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