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HPLC Analysis of Fab/Fc mAb Antibody Fragments on Proteomix® WCX NP1.7

HPLC Analysis of Fab/Fc mAb Antibody Fragments on Proteomix® WCX NP1.7 application for HPLC

Materiales

Analytical column

Referencia del producto
Descripción
Precios

Proteomix® WCX-NP1.7 Weak Cation Exchange Column

L × I.D. 3 cm × 4.6 mm, 1.7 μm particle size, stainless steel

CONDITIONS

column

Proteomix WCX-NP1.7, 3 cm x 4.6 mm I.D., 1.7 μm particles (Z777179)

mobile phase

(A) 20 mM sodium acetate, pH 5.15; (B) A + 1 M LiCl

gradient

12% to 40% B in 6.7 min; to 100% B in 0.1 min; held for 2 min

flow rate

(1.5 mL/min)

pressure

3335-3916 psi (230 - 270 bar)

detector

UV 280 nm

injection

25 μL

sample

1 mg/mL papain digested MAb 321

Descripción

Legal Information

Proteomix is a registered trademark of Sepax Technologies