HPLC Analysis of Cytochrome c Species Variants on Discovery® BIO Wide Pore C18
Materiales
Analytical column
Referencia del producto
Descripción
Precios
Standard
Referencia del producto
Descripción
Precios
Cytochrome c from bovine heart
≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell cultureCitocromo c from equine heart
BioUltra, ≥99% (SDS-PAGE), powder, suitable for mammalian cell cultureCONDITIONS
column
Discovery BIO Wide Pore C18, 15 cm × 4.6 mm I.D., 5 μm particles (568222-U)
mobile phase
[A] (0.1% TFA in water):(0.1% TFA in acetonitrile); (70:30), [B] (0.1% TFA in water):(0.1% TFA in acetonitrile), 64:36
gradient
0-100% B in 30 min
flow rate
1 mL/min
column temp.
ambient
detector
UV, 220 nm
injection
12 μL each at 0.8 mg/mL in 0.1%TFA
Descripción
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany